Chromogenic hydrazide Schiff base reagent: Spectrophotometric determination of CN^- ion

A Schiff base reagent, Picolinohydrazide-naphthol (HL), is used for trace level detection of toxic CN^- selectively in presence of eighteen other anions (SCN^-, OCN^-, S₂O₃^2-, HPO₄^2-, H₂PO₄^-, I^-, ClO₄^-, HSO₄^-, SO₄^2-, AsO₄^3-, NO₂^-, AsO₂^-, Cl^-, F^-, HF₂^-, NO₃^-, Br^-, N₃^-) by visual color change, colorless to yellow, in DMSO/H₂O (9:1, v/v) at pH, 7.2 (HEPES buffer) medium. The sensitivity of the probe shows that the limit of detection (LOD) is 7.08 μM. The probable mechanism for the sensing behavior involves the deprotonation of naphthol-OH by CN^- that has been authenticated by ¹H NMR titration and Mass spectra. The composition (1:1 mol ratio) is supported by Job's plot and binding constant (K_a, 1.5 × 10⁴ M^-1) is reported by Benesi-Hildebrand plot. Furthermore, a simple paper strip device is fabricated for the determination of CN^- ion in water. DFT computation is carried out to explain the electronic spectral feature of the sensor.