Intensification of delignification of sawdust and subsequent enzymatic hydrolysis using ultrasound

The current work investigates intensification of delignification of sawdust and subsequent enzymatic hydrolysis to produce reducing sugars with the use of ultrasound. Alkaline hydrolysis of sawdust was initially performed to remove lignin which hampers the rate of enzymatic hydrolysis. Effect of different parameters in the case of ultrasound (US) assisted and conventional processes such as alkali concentration (0.5-2.5 N), substrate loading (0.2-1.0% w/v) and temperature (40-80 °C) have been investigated. Optimized parameters obtained for US assisted process showed better trends as compared to conventional process with about 1.25 times higher yields and significant reduction in time by about 4 h. The process parameters for US assisted and conventional enzymatic hydrolysis to produce reducing sugars were also optimized by varying substrate loading (0.5-10% w/v), reaction temperature (30-70 °C) along with variation in US power (10-80 W) and US duty cycle (30-90%). US assisted enzymatic hydrolysis performed at 4% w/v substrate loading along with 50 W US power and 50% duty cycle at 50 °C resulted in 7.46 mg/mL of reducing sugars yield within 1 h while conventional stirring with 6% w/v substrate loading and 50 °C resulted in approximately same yield of reducing sugars within 3 h. The requirement of lower time for similar yields or in other words higher yield in same time clearly highlights the process intensification benefits due to the use of ultrasound. Overall it can be concluded from the study that US assisted processes resulted in efficient delignification along with higher yield of reducing sugars in lower treatment time as compared to conventional process.