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Updated: Jan 20, 2026

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
Published on: February 17, 2023
A Coordinated Response at The Transcriptome and Interactome Level is Required to Ensure Uropathogenic Escherichia
Natalia Sanchez de Groot1, Marc Torrent Burgas2
1Gene Function and Evolution Lab, Centre for Genomic Regulation (CRG), Dr. Aiguader 88, 08003 Barcelona, Spain. natalia.sanchez@crg.eu.
Abstract:
Localized infections or disruption of the skin barrier can enable the entry of bacteria into the bloodstream, possibly leading to acute inflammation and sepsis. There is currently no holistic view on how bacteria can survive and spread in the bloodstream. In this context, we combined transposon mutagenesis, gene-expression profiling and a protein interaction network analysis to examine how uropathogenic Escherichia coli can proliferate in blood. Our results indicate that, upon migration from the urea to serum, E. coli reacts to the osmolarity difference, triggering a transcriptomic response in order to express survival genes. The proteins codified by these genes are precisely organized at the interactome level and specifically target short linear motifs located in disordered regions of host proteins. Such a coordinated response helps to explain how bacteria can adapt to and survive environmental changes within the host. Overall, our results provide a general framework for the study of bacteremia and reveal new targets for potential study as novel antimicrobials.
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