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Updated: Jan 20, 2026

Tuning Degradation to Achieve Specific and Efficient Protein Depletion
Published on: July 20, 2019
An efficient auxin-inducible degron system with low basal degradation in human cells
Shiqian Li1,2, Xavier Prasanna3, Veijo T Salo1,2
1Department of Anatomy and Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
Abstract:
Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.
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