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Small Animal Video Tracking for Activity and Path Analysis Using a Novel Open-Source Multi-Platform Application

Srinivasa R Rao1,2, Sam W Z Olechnowicz1,2, Patrick Krätschmer3

  • 1Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.

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Summary

This study introduces AnimApp, a free, open-source software for tracking animal movement. AnimApp reliably quantifies movement parameters, offering a flexible tool for behavioral analysis in research models.

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Area of Science:

  • Biology
  • Ethology
  • Bioinformatics

Background:

  • Manual quantification of animal movement is labor-intensive and prone to error.
  • Existing automated tracking systems are often costly and disruptive to animal models.
  • There is a need for accessible, non-invasive tools for behavioral analysis in experimental biology.

Purpose of the Study:

  • To develop a novel, free, and open-source software application for automated animal movement tracking.
  • To validate the reliability and reproducibility of the developed application for quantifying movement parameters.
  • To provide a flexible and accessible tool for analyzing behavioral changes in various experimental model systems.

Main Methods:

  • Development of a multiplatform application named AnimApp using the OpenCV library.
  • Utilizing computer vision techniques for automated detection and tracking of small animal subjects.
  • Quantification of movement parameters including distance and speed.

Main Results:

  • AnimApp demonstrated reliable and reproducible tracking of small animal movement (rodents, insects).
  • The software successfully quantified key action parameters such as distance and speed.
  • Activity changes induced by handling, environmental enrichment, and temperature alterations were detectable.

Conclusions:

  • AnimApp provides an accurate and reproducible method for analyzing animal movement and behavior.
  • This open-source application offers a cost-effective and flexible alternative to existing tracking systems.
  • AnimApp has broad potential applications across diverse biological model systems for behavioral research.