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Colloids03:22

Colloids

20.8K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
20.8K
Colloids and Suspensions01:17

Colloids and Suspensions

3.1K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
3.1K
Colloidal precipitates01:09

Colloidal precipitates

5.9K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
5.9K
Administering Oxygen by Mask01:30

Administering Oxygen by Mask

2.2K
Administering Oxygen by Mask
Administering oxygen by mask is a common nursing intervention that provides supplemental oxygen to patients with respiratory distress or chronic lung conditions. This procedure involves delivering oxygen at a specified rate through a face mask connected to an oxygen source.
Equipment
The equipment necessary for this procedure includes:
2.2K
Masking and Demasking Agents01:19

Masking and Demasking Agents

3.5K
EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
3.5K
Detection of Black Holes01:10

Detection of Black Holes

2.5K
Although black holes were theoretically postulated in the 1920s, they remained outside the domain of observational astronomy until the 1970s.
Their closest cousins are neutron stars, which are composed almost entirely of neutrons packed against each other, making them extremely dense. A neutron star has the same mass as the Sun but its diameter is only a few kilometers. Therefore, the escape velocity from their surface is close to the speed of light.
Not until the 1960s, when the first neutron...
2.5K

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Related Experiment Video

Updated: Jan 20, 2026

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography
08:21

Fabrication of Periodic Gold Nanocup Arrays Using Colloidal Lithography

Published on: September 2, 2017

7.6K

Single molecule protein patterning using hole mask colloidal lithography.

William Lum1, Dinesh Gautam2, Jixin Chen2

  • 1Department of Chemistry, College of Arts and Sciences, University of Cincinnati, 301 West Clifton Court, Cincinnati OH 45221-0172, USA. saglela@uc.edu.

Nanoscale
|August 28, 2019
PubMed
Summary

This study introduces Hole Mask Colloidal Lithography for precise single protein molecule patterning on surfaces. This technique enables cost-effective, scalable bio-interfacing for advanced biosensors and bioengineered devices.

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Last Updated: Jan 20, 2026

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Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Manipulating single protein molecules on surfaces is crucial for developing advanced bio-interfaced devices.
  • Current methods for precise protein patterning face challenges in cost-effectiveness, scalability, and control over pattern density.
  • Applications span optics, catalysis, bioengineering, and biosensing, requiring high biological activity.

Purpose of the Study:

  • To present a novel, cost-effective technique for single-molecule protein patterning.
  • To demonstrate precise control over feature size, pattern density, and scalability.
  • To enable the development of on-chip devices with enhanced biological functionality.

Main Methods:

  • Hole Mask Colloidal Lithography (HMCL) was employed as a bench-top technique.
  • Glass coverslips were patterned with functional streptavidin protein in feature sizes ranging from 15-200 nm.
  • Atomic Force Microscopy (AFM) and single-molecule fluorescence microscopy were used for characterization and validation.

Main Results:

  • HMCL successfully patterned functional streptavidin onto glass coverslips with tunable feature sizes (15-200 nm) and variable pitch.
  • AFM confirmed the size of the patterned features.
  • Single-molecule fluorescence microscopy validated tunable pattern density and confirmed the presence of functional, patterned streptavidin molecules.

Conclusions:

  • Hole Mask Colloidal Lithography offers a scalable and cost-effective solution for single-molecule protein patterning.
  • This technique facilitates the creation of highly active on-chip bio-devices.
  • The demonstrated control over patterning is vital for advancing biosensing and bioengineering applications.