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Related Experiment Videos

The human Lyt-3 molecule requires CD8 for cell surface expression.

J P DiSanto1, R W Knowles, N Flomenberg

  • 1Effector Lymphocyte Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.

The EMBO Journal
|November 1, 1988
PubMed
Summary
This summary is machine-generated.

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Researchers identified the human Lyt-3 molecule using the T8/2T8-5H7 antibody. This molecule requires Lyt-2 for cell surface expression, suggesting a role in T cell interactions.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • A previously identified monoclonal antibody, T8/2T8-5H7, showed CD8-like clustering but did not react with human CD8 (Lyt-2 homologue) transfectants.
  • This suggested T8/2T8-5H7 might target a different molecule, possibly the human Lyt-3 gene product.

Purpose of the Study:

  • To investigate the hypothesis that T8/2T8-5H7 recognizes the human Lyt-3 molecule.
  • To characterize the human Lyt-3 molecule and its expression requirements.

Main Methods:

  • Isolation of a full-length cDNA encoding the human Lyt-3 molecule.
  • Characterization of the Lyt-3 gene product through transfection experiments.

Main Results:

  • The human Lyt-3 cDNA encodes a protein product recognized by the T8/2T8-5H7 antibody.

Related Experiment Videos

  • Efficient cell surface expression of human Lyt-3 requires co-expression with the human Lyt-2 homologue.

    • Conclusions:

    • The antibody T8/2T8-5H7 specifically recognizes the human Lyt-3 molecule.
    • Human Lyt-3 and Lyt-2 form a heterodimer essential for cell surface expression, potentially playing a role in T cell-target cell interactions.