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PTPRO exaggerates inflammation in ulcerative colitis through TLR4/NF-κB pathway.

Jie Zhao1,2, Shushan Yan3, Xianlan Zhu4

  • 1Department of General Surgery, The Affiliated Jiangning Hospital with Nanjing Medical University, Nanjing, China.

Journal of Cellular Biochemistry
|August 28, 2019
PubMed
Summary

Protein tyrosine phosphatase receptor type O (PTPRO) exacerbates ulcerative colitis (UC) inflammation by promoting toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) signaling. PTPRO is also a potential diagnostic marker for UC.

Keywords:
nuclear factor κBprotein tyrosine phosphatase receptor type Otoll-like receptor 4ulcerative colitis

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Area of Science:

  • Immunology
  • Gastroenterology
  • Molecular Biology

Background:

  • Ulcerative colitis (UC) is a chronic inflammatory bowel disease with incompletely understood mechanisms.
  • Protein tyrosine phosphatase receptor type O (PTPRO) is implicated in inflammatory diseases, but its role in UC is unclear.

Purpose of the Study:

  • To investigate the role and mechanism of PTPRO in ulcerative colitis (UC).
  • To evaluate PTPRO as a potential diagnostic marker for UC.

Main Methods:

  • Analysis of PTPRO, TLR4, and cytokine expression in UC patient tissues and cells.
  • In vitro studies using lipopolysaccharide (LPS)-induced macrophage models with PTPRO manipulation.
  • Assessment of intestinal epithelial cell (IEC) proliferation and apoptosis.
  • Evaluation of NF-κB signaling pathway activation.
  • Receiver operating characteristic (ROC) curve analysis for diagnostic potential.

Main Results:

  • PTPRO, TLR4, and inflammatory cytokine expression were elevated in UC tissues and cells.
  • PTPRO promoted TLR4 and cytokine expression in LPS-induced macrophages.
  • PTPRO inhibited IEC proliferation and enhanced IEC apoptosis.
  • PTPRO overexpression increased phosphorylated NF-κB/p65 and IκBα levels.
  • PTPRO demonstrated significant diagnostic potential for UC (AUC = 0.807).

Conclusions:

  • PTPRO exacerbates UC inflammation through the TLR4/NF-κB signaling pathway.
  • PTPRO represents a promising diagnostic biomarker for ulcerative colitis.