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lncRNA LINC-PINT is downregulated in melanoma and regulates cell proliferation by downregulating lncRNA BANCR

Qing Huang1,2, Deli Zhang3,4, Qingchun Diao3

  • 1Department of Dermatology, Chongqing Shapingba District People's Hospital, Chongqing 400011, P.R. China.

Oncology Letters
|August 28, 2019
PubMed

Insights

Long non-coding RNA LINC-PINT is downregulated in melanoma and inhibits cancer cell growth. It appears to regulate melanoma proliferation by decreasing BANCR, another long non-coding RNA.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play roles in various cancers, but their functions in melanoma are largely uncharacterized.
  • Investigating specific lncRNAs like LINC-PINT is crucial for understanding melanoma development.

Purpose of the Study:

  • To investigate the role of long intergenic non-protein coding RNA p53 induced transcript (LINC-PINT) in melanoma.
  • To explore the relationship between LINC-PINT, BRAF-activated non-coding RNA (BANCR), and melanoma cell proliferation.

Main Methods:

  • Quantitative PCR (qPCR) for gene expression analysis.
  • Overexpression experiments to study gene interactions.
  • Cell Counting Kit-8 (CCK-8) assays for cell proliferation assessment.

Main Results:

  • LINC-PINT was found to be downregulated in melanoma tissues, inversely correlating with tumor thickness.
  • BANCR expression was upregulated in melanoma and showed a positive correlation with tumor thickness.
  • LINC-PINT overexpression inhibited melanoma cell proliferation and downregulated BANCR expression.

Conclusions:

  • LINC-PINT is downregulated in melanoma and acts as a potential tumor suppressor.
  • LINC-PINT may regulate melanoma cell proliferation by downregulating BANCR expression.
Keywords:
long non-coding BANCRlong non-coding RNA LINC-PINTmelanomaproliferation

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