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blaNDM-5 carried by a hypervirulent Klebsiella pneumoniae with sequence type 29

Yi Yuan1, Ying Li2, Guangxi Wang3

  • 1Department of Laboratory Medicine, The First People's Hospital of Neijiang, Neijiang, Sichuan China.

Antimicrobial Resistance and Infection Control
|August 28, 2019
PubMed
Abstract

Insights

A carbapenem-resistant, hypervirulent Klebsiella pneumoniae strain (ST29) carrying NDM-5 was identified. This hypervirulent Klebsiella pneumoniae highlights the need for surveillance of this resistant pathogen.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • A carbapenem-resistant, hypermucoviscous Klebsiella pneumoniae isolate was recovered from human sputum.
  • This isolate exhibited hypervirulence and carried the blaNDM-5 gene.

Purpose of the Study:

  • To characterize the clonal background, antimicrobial resistance, and virulence factors of a carbapenem-resistant hypermucoviscous Klebsiella pneumoniae isolate.
  • To investigate the evolution and transfer of the blaNDM-5 gene within IncX3 plasmids.

Main Methods:

  • Whole genome sequencing of the Klebsiella pneumoniae isolate.
  • Virulence assays using wax moth larvae.
  • Conjugation experiments to assess gene transfer.
  • Phylogenetic analysis of ST29 Klebsiella pneumoniae and IncX3 plasmids.

Main Results:

  • The isolate, belonging to sequence type 29 (ST29), carried a virulence plasmid (pVir-SCNJ1) with the rmpA gene, conferring high virulence.
  • The blaNDM-5 gene was located on a conjugative IncX3 plasmid (pNDM5-SCNJ1).
  • Phylogenetic analysis suggested multiple acquisition events of blaNDM-5 in IncX3 plasmids.

Conclusions:

  • Characterization of a blaNDM-5-positive hypervirulent Klebsiella pneumoniae ST29 strain in China.
  • The findings underscore the importance of active surveillance for this lineage of carbapenem-resistant Klebsiella pneumoniae.
Keywords:
Carbapenem resistanceHypervirulentIncX3blaNDM-5

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