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Updated: Jan 20, 2026

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis
Published on: November 12, 2015
Identifying crossing collagen fibers in human corneal tissues using pSHG images
M Alizadeh1,2,3, D Merino2,4,3, G Lombardo5,6
1Department of Physics, University of Kurdistan, Pasdaran St., 66177-15177, Sanandaj, Iran.
Abstract:
Polarization sensitive second harmonic generation (pSHG) microscopy has been used previously to characterize the structure of collagen fibers in corneal samples. Due to the typical organization of the corneal stroma, the information that pSHG provides may be misleading in points where two different collagen fiber bundles orient along different direction crossings. Here, a simulation that illustrates the problem is presented, along with a novel method that is capable of identifying these crossing points. These results can be used to improve the evaluation of corneal collagen structure, and it has been applied to analyze pSHG data acquired from healthy and keratoconic human corneal samples.
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