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Updated: Jan 20, 2026

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
Published on: February 26, 2015
ChIP and Chips: Introducing the WormPharm for correlative studies employing pharmacology and genome-wide analyses in
Jung H Doh1, Andrew B Moore2, İrem Çelen3
1University of Delaware, Delaware Biotechnology Institute, Newark, DE, USA.
Abstract:
We present the WormPharm, an automated microfluidic platform that utilizes an axenic medium to culture C. elegans. The WormPharm is capable of sustaining C. elegans for extended periods, while recording worm development and growth with high temporal resolution ranging from seconds to minutes over several days to months. We demonstrate the utility of the device to monitor C. elegans growth in the presence of varying doses of nicotine and alcohol. Furthermore, we show that C. elegans cultured in the WormPharm are amendable for high-throughput genomic assays, i.e. chromatin-immunoprecipitation followed by next generation sequencing, and confirm that nematodes grown in monoxenic and axenic cultures exhibit genetic modifications that correlate with observed phenotypes. The WormPharm is a powerful tool for analyzing the effects of chemical, nutritional and environmental variations on organism level responses in conjunction with genome-wide changes in C. elegans.
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