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Updated: Jan 20, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
Published on: March 14, 2018
Easy and low-cost stable positioning of suspension cells during live-cell imaging
Daniel Ivanusic1, Kazimierz Madela1, Joachim Denner1
1Robert Koch Institute, Berlin, Germany.
Abstract:
Dynamic processes of cells can be best monitored when living cells are analyzed by imaging. While it is easy to observe adherent living cells it has been extremely challenging to analyze suspension cells. This cell type floats freely in the culture dish, and it is only a question of time when the focus or the observation field is lost. In order to keep the cells in focus, an easy and inexpensive method allowing the observation of living suspension cells during confocal laser scanning microscope imaging was developed.
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