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Related Experiment Video

Updated: Jan 20, 2026

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
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A rapid, high-throughput method for determining chronological lifespan in budding yeast.

Zachery R Belak1, Troy Harkness2, Christopher H Eskiw1,2

  • 1Department of Food and Bioproduct Sciences, College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SaskatchewanS7N 5A8, Canada.

Journal of Biological Methods
|August 28, 2019
PubMed
Summary

A new MTT-based assay accurately measures yeast chronological lifespan, offering a faster, cost-effective alternative to traditional methods for aging research.

Keywords:
MTT assaySaccharomyces cerevisiaecaloric restrictionchronological lifespan

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Area of Science:

  • * Gerontology and cellular aging research.
  • * Model organism studies in Saccharomyces cerevisiae.

Background:

  • * Budding yeast Saccharomyces cerevisiae is a key model organism for aging studies.
  • * Yeast lifespan is influenced by caloric restriction and pharmacological interventions, similar to metazoans.
  • * Traditional chronological lifespan assays are reliable but labor-intensive and costly.

Purpose of the Study:

  • * To develop and validate a novel MTT-based method for assessing yeast chronological lifespan.
  • * To establish a high-throughput assay for screening anti-aging compounds and yeast strains.
  • * To provide a more efficient and cost-effective alternative to traditional lifespan assays.

Main Methods:

  • * Development of a novel MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)-based assay for yeast chronological lifespan.
  • * Comparison of the MTT assay with traditional colony counting methods.
  • * Validation using caloric restriction and known long-lived/short-lived yeast strains.
  • * Adaptation of the MTT assay into a high-throughput screening platform.

Main Results:

  • * The MTT-based method demonstrates rigorous and reliable measurement of yeast lifespan.
  • * The assay accurately detects lifespan extension due to caloric restriction.
  • * It effectively distinguishes between long-lived and short-lived yeast strains.
  • * The high-throughput version allows for rapid screening of potential anti-aging agents and strains.

Conclusions:

  • * The novel MTT-based assay is a valid and efficient alternative to traditional methods for measuring yeast chronological lifespan.
  • * This high-throughput method accelerates the identification of anti-aging compounds and genetic factors influencing lifespan.
  • * The findings may facilitate the discovery of therapeutic agents for aging in higher animals, including humans.