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Related Experiment Video

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Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
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Real time mitochondrial dimension measurements.

Joseph M Leichner1, Evgene Konyukhov1, David Kamoun1

  • 1Biomedical Engineering Faculty, Technion-IIT, Haifa 32000, Israel.

Journal of Biological Methods
|August 28, 2019
PubMed
Summary

Researchers developed new software to measure mitochondrial 2D deformation in cardiomyocytes in real time. This tool aids in understanding cell function and cardiac disease progression.

Keywords:
energeticsmitochondrial deformationmitochondrial volumereal timesarcomere length

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Area of Science:

  • Cell Biology
  • Biophysics
  • Cardiovascular Research

Background:

  • Mitochondrial volume changes correlate with cell function and cardiac disease.
  • Current methods for measuring mitochondrial dimensions are limited (isolated mitochondria, short/toxic measurements).
  • Fourier transformation of transmitted light intensity variations can measure mitochondrial deformation along one axis.

Purpose of the Study:

  • To develop an open-source program for real-time, 2D mitochondrial deformation quantification in cardiomyocytes.
  • To overcome limitations of existing methods for measuring mitochondrial dimensions in situ.
  • To provide a tool for studying cell biophysics, bioenergetics, and cardiac disease.

Main Methods:

  • Designed an open-source LabVIEW program utilizing transmitted light diffraction.
  • Quantified mitochondrial two-dimension (2D) deformation in cardiomyocytes in situ.
  • Validated the program on synthetic and experimental images from rabbit and rat ventricular myocytes.

Main Results:

  • The program enables real-time, long-period (seconds) analysis of 2D mitochondrial deformation.
  • Simultaneous analysis of mitochondrial and sarcomere length dynamics is possible.
  • The program accurately analyzes images from various cameras.

Conclusions:

  • This novel program offers a powerful tool for exploring cell biophysics and bioenergetics.
  • Real-time 2D mitochondrial deformation quantification is feasible and valuable.
  • This technology may form the basis for future clinical tools to assess cardiac disease.