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Related Experiment Videos

Improved heart preservation with UW preservation solution.

D K Swanson1, I Pasaoglu, H A Berkoff

  • 1Division of Thoracic and Cardiovascular Surgery, University of Wisconsin Hospital and Clinics, Madison.

The Journal of Heart Transplantation
|November 1, 1988
PubMed
Summary
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The UW solution effectively preserves canine hearts for up to 12 hours of cold storage, demonstrating superior recovery of ventricular function and tissue homeostasis compared to other preservation methods for heart transplantation.

Area of Science:

  • Cardiology
  • Transplantation Biology
  • Organ Preservation

Background:

  • Current heart preservation techniques require improvement for extended storage.
  • The University of Wisconsin (UW) solution has shown success in preserving other organs like the pancreas, kidney, and liver.
  • Evaluating UW solution's efficacy for cardiac preservation is crucial for advancing heart transplantation.

Purpose of the Study:

  • To assess the efficacy of the UW solution in preserving canine hearts for 5 and 12 hours.
  • To compare UW solution's performance against Stanford and modified Collins' solutions for heart preservation.
  • To determine the impact of different preservation solutions on post-ischemic cardiac function and tissue integrity.

Main Methods:

  • Canine hearts were preserved using UW, Stanford, or modified Collins' solutions for 5 or 12 hours at 4°C.

Related Experiment Videos

  • Hearts were reperfused in an isolated working heart preparation to evaluate ventricular function.
  • Adenosine triphosphate (ATP) content, tissue electrolytes, water content, lactate, and oxygen utilization were measured.

    • Main Results:

    • Hearts preserved with UW solution for 5 hours showed near-normal ventricular function and ATP levels.
    • After 12 hours, UW solution-preserved hearts exhibited rapid recovery of left ventricular function and normalized tissue water and sodium content.
    • Stanford solution provided inadequate preservation, while modified Collins' solution showed slower recovery compared to UW.

    Conclusions:

    • The UW solution offers superior heart preservation compared to Stanford and modified Collins' solutions, especially for extended storage periods.
    • UW solution facilitates rapid functional recovery and maintains tissue homeostasis in hearts after 12 hours of cold ischemia.
    • The UW solution warrants further investigation for potential clinical application in heart transplant programs.