Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Caspases01:24

Caspases

13.7K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
13.7K
The Retina01:32

The Retina

74.8K
The retina is a layer of nervous tissue at the back of the eye that transduces light into neural signals. This process, called phototransduction, is carried out by rod and cone photoreceptor cells in the back of the retina.
74.8K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

6.7K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
6.7K
Physiological Barriers01:25

Physiological Barriers

5.1K
Physiological barriers are semi-permeable cellular structures restricting drug diffusion into intracellular compartments and tissues. There are six types of physiological barriers: blood endothelial, cell membrane, blood-brain, blood-cerebrospinal fluid (CSF), blood-placenta, and blood-testis barriers.
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...
5.1K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.4K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.4K
Liver Physiology01:30

Liver Physiology

3.5K
The liver, an essential organ in the human body, performs over 200 vital functions that can be broadly categorized into metabolic, hematological, endocrine regulation, and bile production.
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of  70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reduced platelet count in mice protects against glucose intolerance and beta cell loss during a long-term high-fat diet.

Frontiers in immunology·2026
Same author

Long-term antigen reduction does not achieve durable HBV control in mice but enhances therapeutic vaccine efficacy.

JHEP reports : innovation in hepatology·2026
Same author

HIRA-mediated H3.3 deposition preserves hepatocyte cell identity during liver aging.

Research square·2026
Same author

The hepatitis E virus capsid protein forms amyloid-like fibrils that sequester TANK-binding kinase 1 to dampen antiviral responses.

Cell reports·2026
Same author

Spatial distribution and prognostic value of tumor-associated macrophages in head and neck squamous cell carcinomas.

Frontiers in oncology·2026
Same author

Blood plasma and oral rinse liquid profiling for human papillomavirus in head and neck cancer - Unmasking false-positive p16 tissue cases and tracking disease dynamics.

Journal of translational medicine·2026

Related Experiment Video

Updated: Jan 20, 2026

Rat Mesentery Angiogenesis Assay
18:30

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

14.2K

Caspase-8 modulates physiological and pathological angiogenesis during retina development.

Nathalie Tisch1,2,3, Aida Freire-Valls1,4, Rosario Yerbes1,5

  • 1Biochemistry Center.

The Journal of Clinical Investigation
|August 28, 2019
PubMed
Summary

Caspase-8 (Casp-8) in endothelial cells is crucial for blood vessel formation in the retina. Its absence hinders vessel growth but reduces pathological neovascularization in models of retinopathy.

Keywords:
AngiogenesisApoptosis pathwaysCaspases and caspase substratesRetinopathy

More Related Videos

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
08:47

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Published on: July 9, 2013

47.1K
In vivo Electroporation of Developing Mouse Retina
05:53

In vivo Electroporation of Developing Mouse Retina

Published on: June 24, 2011

21.5K

Related Experiment Videos

Last Updated: Jan 20, 2026

Rat Mesentery Angiogenesis Assay
18:30

Rat Mesentery Angiogenesis Assay

Published on: June 18, 2011

14.2K
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
08:47

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Published on: July 9, 2013

47.1K
In vivo Electroporation of Developing Mouse Retina
05:53

In vivo Electroporation of Developing Mouse Retina

Published on: June 24, 2011

21.5K

Area of Science:

  • Vascular biology
  • Cell death signaling
  • Developmental biology

Background:

  • Blood vessel formation (angiogenesis) is essential for development and requires precise regulation.
  • The role of cell death signaling molecules, like Caspase-8 (Casp-8), in angiogenesis is not fully understood.
  • Casp-8 mediates apoptosis and necroptosis, key components of cell death pathways.

Purpose of the Study:

  • To investigate the function of Casp-8 in endothelial cells (ECs) during postnatal retinal angiogenesis.
  • To determine if Casp-8's role in angiogenesis is dependent on its cell death functions.
  • To explore the therapeutic potential of targeting Casp-8 in retinopathy models.

Main Methods:

  • Generated endothelial cell-specific Casp-8 knockout (Casp-8ECKO) mice.
  • Assessed retinal angiogenesis in newborn Casp-8ECKO mice.
  • Utilized an oxygen-induced retinopathy (OIR) mouse model.
  • Analyzed EC proliferation, sprouting, migration, and molecular signaling pathways (MAPK, VE-cadherin).

Main Results:

  • Casp-8ECKO mice exhibited impaired retinal angiogenesis, with reduced EC proliferation, sprouting, and migration.
  • Loss of Casp-8 led to hyperactivation of p38 MAPK and destabilization of VE-cadherin at EC junctions, independent of cell death.
  • In the OIR model, Casp-8 deletion in ECs reduced pathological neovascularization.

Conclusions:

  • Casp-8 plays a critical, cell death-independent role in regulating retinal vascular development.
  • Casp-8 signaling in ECs is implicated in the pathophysiology of retinopathy of prematurity (ROP).
  • Targeting Casp-8 in ECs may offer a therapeutic strategy for ROP and related conditions.