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Published on: October 13, 2020
Analysis of neurotransmitters in Daphnia magna affected by neuroactive pharmaceuticals using liquid
Cristian Gómez-Canela1, Xavier Rovira García2, Fernando Martínez-Jerónimo3
1Department of Analytical Chemistry and Organic Chemistry, Campus Sescelades, Faculty of Chemistry, Universitat Rovira i Virgili, Marcel∙lí Domingo s/n, Tarragona, Catalonia, Spain.
Abstract:
Neurotransmission plays an essential role during the central nervous system (CNS) development. During the last years, several studies based on the changes produced in neurotransmitters of aquatic organisms caused by pharmaceuticals have been reported. Daphnia magna, the aquatic ecotoxicological model organism, shares several of the neurotransmitters targeted by antidepressant and other neuro-active drugs with vertebrates. Therefore, a method based on liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) has been applied for the first time to study the levels of 41 neurotransmitters in Daphnia magna under the effect of four different neuro-active pharmaceuticals (sertraline, venlafaxine, duloxetine and fluoxetine). In addition, the performance of LC-HRMS was studied in terms of linearity, sensitivity, intra- and inter-day precision, and overall robustness. The developed analytical method using LC-HRMS is a new tool for neurotoxicology research using the Daphnia magna model. As a result, general differences on the concentrations of those neurotransmitters exposed to the mentioned pharmaceuticals were observed.
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