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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
Published on: September 29, 2017
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Two types of polyelectrolyte multilayers hydrogel membrane based on chitosan and alginate with different
Guoshuang Zheng1, Xiudong Liu2, Minghui Hu1
1Laboratory of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning, China; National-Local Joint Engineering Laboratory for the Development of Orthopedic Implant Materials, Dalian, Liaoning, China.
International Journal of Biological Macromolecules
|August 28, 2019
Summary
Researchers engineered chitosan and alginate hydrogel membranes using layer-by-layer assembly. Surface roughness, not stiffness, controlled cell adhesion, enabling tailored biomaterial surfaces for tissue engineering.
Area of Science:
- Biomaterials Science
- Materials Chemistry
- Tissue Engineering
Background:
- Controlling mammalian cell adhesion at material interfaces is crucial for tissue engineering.
- Surface properties of biomaterials significantly influence cell adhesion and behavior.
- Marine-derived polysaccharides like chitosan (CHI) and alginate (ALG) offer versatile building blocks for biomaterials.
Purpose of the Study:
- To fabricate and characterize polyelectrolyte multilayers (PEMs) hydrogel membranes using CHI and ALG.
- To investigate the effect of assembly sequence and chitosan degree of deacetylation (DD) on PEM properties.
- To evaluate the influence of PEM surface properties on mouse fibroblast (L929) cell adhesion.
Main Methods:
- Fabrication of ALG/CHI PEMs hydrogel membranes via the layer-by-layer (LbL) technique.
- Systematic variation of the assembly sequence of CHI with different DD.
- Characterization of PEM surface properties (roughness, morphology) and assessment of L929 cell adhesion.
Main Results:
- PEM formation was influenced by the rigidity and conformation of chitosan molecules.
- L929 cell adhesion was found to be critically dependent on surface roughness, not surface stiffness.
- PEM surfaces could be engineered to be either strongly cell-adhesive (cytophilic) or cell-resistant (cytophobic).
Conclusions:
- ALG/CHI PEMs offer tunable surface properties through controlled fabrication.
- Surface roughness is a key factor in modulating cell behavior on these biomaterials.
- These engineered membranes show potential for manipulating cell interactions in tissue-engineered constructs.
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