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Updated: Jan 20, 2026

Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
Tracking the polymerization of DNA sensors and inflammasomes using FRET
Zachary Mazanek1, Jungsan Sohn1
1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Abstract:
Upon activation, DNA sensors and inflammasomes often polymerize into filamentous oligomers, and transduce the incoming pathogenic signal via inducing the assembly of downstream filaments. Given the complexity of these supramolecular structures, kinetics and thermodynamics that govern their assembly mechanisms remain poorly understood. Here, we present a simple yet robust assay that can track the assembly of these filaments in a quantitative manner. This FRET-based measurement is cost effective and also amenable to high-throughput screening.
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