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Related Experiment Video

Updated: Jan 20, 2026

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
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Single-Molecule Surface Plasmon-Coupled Emission with Plasmonic Gratings.

Aaron Wood1, Cherian J Mathai2, Keshab Gangopadhyay2

  • 1Bioengineering Department, University of Missouri, 254 Agricultural Engineering, 65211-5200 Columbia, Missouri, United States.

ACS Omega
|August 29, 2019
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Summary

Scientists can now image single molecules (SM) using surface plasmon-coupled emission and a DVD grating. This technique images and identifies fluorophores by their unique angular emission patterns, advancing SM imaging capabilities.

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Area of Science:

  • Optics and Photonics
  • Nanotechnology
  • Spectroscopy

Background:

  • Imaging single molecules (SM) is a long-standing scientific goal.
  • Advances in microscopy enable routine observation of individual fluorescent molecules.
  • Surface plasmon resonance (SPR) enhances SM emission but has limitations.

Purpose of the Study:

  • To develop a novel method for imaging and identifying individual fluorophores.
  • To leverage the full potential of plasmonic properties for enhanced SM imaging.
  • To utilize surface plasmon-coupled emission for angularly resolved SM analysis.

Main Methods:

  • Employing surface plasmon resonance (SPR) excitation.
  • Utilizing surface plasmon-coupled emission from a high-definition digital versatile disc (DVD) grating.
  • Analyzing the angular emission patterns of individual molecules to identify fluorophores.

Main Results:

  • Successful imaging and identification of different fluorophores using angular emission.
  • Demonstrated the effectiveness of SPR excitation and coupled emission from a DVD grating.
  • Showcased a method that exploits unique plasmonic properties for SM imaging.

Conclusions:

  • The developed technique significantly improves single molecule imaging capabilities.
  • This method has broad implications for multiplexed single molecule spectroscopy.
  • The findings pave the way for advanced single molecule fluorescence imaging applications.