Related Experiment Video
Updated: Jan 20, 2026

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Published on: October 8, 2013
Fluorescence Imaging of Disrupted Interfaces between Liquid-Ordered and Liquid-Disordered Domains by a Flavin-Labeled
1Frontier Research Core for Life Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Abstract:
Lipid rafts and membrane-active peptides are attracting attention because they help understand basic membrane functions. In addition, we focus on flavoproteins playing some physiological roles and explore the model compounds. In this study, we demonstrate that a new flavin probe, composed of palmitoylated peptide nucleic acid (PNA) and its complementary PNA labeled with flavin, targets the liquid-ordered (lo) microdomains and disrupts its interfaces to liquid-disordered (ld) microdomains of giant unilamellar vesicles and can be visualized by using confocal laser scanning microscopy. Surprisingly, as shown in time-lapse images, vesiculation and probe aggregations appear in the lo-ld interfaces, which leads to local disruption of the membrane. We discuss a possible interpretation of the data based on a comparison with control experiments.
Related Concept Videos
10:38Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Efficiency of Liquid-liquid Extraction
Liquid-liquid extraction (LLE) is a separation technique used instead of distillation when either: (a) the relative volatilities of the compounds to be separated are very similar; (b) one or more of the mixture components are temperature sensitive even near ambient conditions; (c) the distillation would require a very low pressure or a very high distillate/feed...
13:38Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures
05:26A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
04:10Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes
10:47Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
