Related Experiment Video
Updated: Jan 20, 2026

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
Published on: September 7, 2011
Gram-Scale Laboratory Synthesis of TC AC 28, a High-Affinity BET Bromodomain Ligand
Raysa Khan1, Graham Marsh2, Robert Felix2
1Department of Chemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QJ, U.K.
Abstract:
TC AC 28, 6-(1H-Indol-4-yl)-8-methoxy-1-methyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine-4-acetic acid methyl ester, has been synthesized on a near-gram scale in seven steps with notable improvements in the reported poor-yielding last two steps enabling this key chemical probe compound to be available for researchers.
Related Concept Videos
08:45Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
08:34MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
09:15Synthesis of Ligand-free CdS Nanoparticles within a Sulfur Copolymer Matrix
09:45Accessing Valuable Ligand Supports for Transition Metals: A Modified, Intermediate Scale Preparation of 1,2,3,4,5-Pentamethylcyclopentadiene
03:23Mobility Shift Affinity Capillary Electrophoresis: A Method to Analyze Sample-Ligand Interactions Depending on Differential Migration of Protein-Ligand Complexes
Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...

