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Updated: Jan 20, 2026
Synthesis of Luminol and Chemiluminescence - Concept
Sensitive and Selective Carmine Acid Detection Based on Chemiluminescence Quenching of Layer Doubled
Feng Pan1,2, Yilin Zhang1, Zhiqin Yuan2
1School of Environment, Key Laboratory for Yellow Riverand Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University, Xinxiang, Henan 453007, China.
Abstract:
Carminic acid (CA) extracted from cochineal is widely used in food additives as a natural colorant, and its potential risk to human health makes its detection important. In this work, a layered doubled hydroxide (LDH)-luminol-H2O2 system-based chemiluminescence (CL) platform has been successfully applied for CA sensing. The principle detection consists of two steps: first, LDH adsorbs CA onto the surface via electrostatic attraction; second, CA quenches the CL of the LDH-luminol-H2O2 system via the synergistic effect of CL resonance energy transfer, reduction of reactive oxygen species, and occupation of positively charged centers of brucite-like layers. With this CL approach, 0.5 μM CA is detectable using a CL spectrometer, and the limit of detection is 0.03 μM. This CL system exhibited a linear response to CA in the concentration range from 0.5 to 10 μM. In addition, the practical application of the designed CL sensing system is evaluated with dried pork slice samples.
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