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Updated: Jan 20, 2026

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
Published on: February 12, 2022
Analysis of Egg White Protein Composition with Double Nanohole Optical Tweezers
Noa Hacohen1, Candice J X Ip1, Reuven Gordon1
1Faculty of Engineering, Department of Electrical and Computer Engineering, University of Victoria, Victoria, British Columbia V8W 3P6, Canada.
Abstract:
We use a double nanohole optical tweezer to analyze the protein composition of egg white through analysis of many individual protein trapping events. The proteins are grouped by mass based on two metrics: standard deviation of the trapping laser intensity fluctuations from the protein diffusion and the time constant of these fluctuations coming from the autocorrelation. Quantitative analysis is demonstrated for artificial samples, and then, the approach is applied to real egg white. The composition found from real egg white corresponds well to past reports using gel electrophoresis. This approach differs from past works by allowing for individual protein analysis in heterogeneous solutions without the need for denaturing, labeling, or tethering.
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