Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

49.9K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
49.9K
Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study10:10

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

10.7K
The motion regimes of ibuprofen encapsulated in β-cyclodextrin nanosponges polymer network are investigated using pulsed-field-gradient spin-echo (PGSE) NMR technique. Synthesis, purification, drug loading, implementation of the NMR pulse sequence and data analysis to work out the mean square displacement of the drug at several observation times are described in...
10.7K
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry08:51

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

598
Here, we present a detailed protocol for the release of O-glycans from mucins, and the subsequent desalting, permethylation, and analysis using MALDI-TOF mass...
598
Physical Properties Of Minerals I: Crystals and Cleavage07:33

Physical Properties Of Minerals I: Crystals and Cleavage

53.4K
Source: Laboratory of Alan Lester - University of Colorado Boulder
The physical properties of minerals comprise various measurable and discernible attributes, including color, streak, magnetic properties, hardness, crystal growth form, and crystal cleavage. Each of these properties are mineral-specific, and they are fundamentally related to a particular mineral’s chemical make-up and atomic structure.
This experiment examines two properties that stem primarily from symmetric repetition of...
53.4K
A Fluorogenic Peptide Cleavage Assay to Screen the Proteolytic Activity of Proteases02:42

A Fluorogenic Peptide Cleavage Assay to Screen the Proteolytic Activity of Proteases

566
This video demonstrates an assay to screen for the proteolytic activity of proteases using fluorogenic peptides. The protease recognizes its cleavage site on the peptide, cleaving it and separating the quencher from the fluorophore, enabling its fluorescence emission. The fluorescence signal is detected and analyzed to check for the cleavage efficiency of different peptide...
566
Oxidative Cleavage of Alkenes: Ozonolysis01:46

Oxidative Cleavage of Alkenes: Ozonolysis

12.8K
In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrostatic pressure spectroscopy reveals the adaptation of microbial rhodopsins to high-pressure environment.

Scientific reports·2026
Same author

A polymorphism and expression levels of SCD and their relationships to production traits in a Japanese farmed emu population.

PloS one·2026
Same author

Oblique-View Method Improves the Detection of cement leakage in Cement-Augmented Pedicle Screw Fixation.

Spine surgery and related research·2026
Same author

Supramolecular colorimetric pressure sensing: ratiometric quantification based on pressure-modulated association.

Chemical communications (Cambridge, England)·2026
Same author

An unusual presentation of dialysis-related amyloidosis: a large tumor extending from the gingiva to the palate.

CEN case reports·2026
Same author

Adaptation-dependent modulation of coordinated muscle activities in human gait adaptation as revealed by tensor decomposition.

iScience·2026

Related Experiment Video

Updated: Jan 20, 2026

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
10:10

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

10.7K

Efficient Cleavage of Permethylated Cyclodextrins.

Yuki Ishida1, Gaku Fukuhara2,3

  • 1Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan.

ACS Omega
|August 29, 2019
PubMed
Summary
This summary is machine-generated.

Optimizing reaction time improves the cleavage of permethylated cyclodextrins. This method efficiently yields maltooctaose derivatives, valuable synthetic building blocks.

More Related Videos

Embryogenesis: Cleavage and Blastulation
01:33

Embryogenesis: Cleavage and Blastulation

49.9K
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
08:51

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Published on: June 20, 2025

598

Related Experiment Videos

Last Updated: Jan 20, 2026

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
10:10

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Published on: August 15, 2016

10.7K
Embryogenesis: Cleavage and Blastulation
01:33

Embryogenesis: Cleavage and Blastulation

49.9K
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
08:51

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Published on: June 20, 2025

598

Area of Science:

  • Carbohydrate Chemistry
  • Organic Synthesis
  • Analytical Chemistry

Background:

  • Permethylated cyclodextrins are key carbohydrate structures.
  • Efficient synthesis of cyclodextrin derivatives is crucial for chemical applications.
  • Controlling cleavage reactions impacts product yield and purity.

Purpose of the Study:

  • To investigate the effect of reaction time on cyclodextrin cleavage.
  • To optimize conditions for producing maltooctaose derivatives.
  • To establish a new synthetic route for valuable carbohydrate building blocks.

Main Methods:

  • Permethylated cyclodextrin cleavage reactions.
  • Optimization of reaction parameters, focusing on time.
  • Analysis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

Main Results:

  • Reaction time significantly influences the purity and yield of maltohexaose derivatives.
  • Optimized conditions led to the efficient cleavage of permethylated gamma-cyclodextrin.
  • A maltooctaose derivative was successfully synthesized in high yield.

Conclusions:

  • Reaction time is a critical factor in permethylated cyclodextrin cleavage.
  • MALDI-TOF MS with synthetic data aids in optimizing cleavage reactions.
  • The developed method provides a high-yield route to maltooctaose derivatives for synthetic chemistry.