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We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.
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The present protocol describes a convenient approach to integrating optical trapping and surface-enhanced Raman spectroscopy (SERS) to manipulate plasmonic nanoparticles for sensitive molecular detection. Without aggregating agents, the trapping laser assembles plasmonic nanoparticles to enhance the SERS signals of target analytes for in situ spectroscopic...
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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Ag/Au Nanoparticle-Loaded Paper-Based Versatile Surface-Enhanced Raman Spectroscopy Substrates for Multiple

Sree Satya Bharati Moram1, Chandu Byram1, Sini Nanadath Shibu1

  • 1Advanced Centre of Research in High Energy Materials (ACRHEM), University of Hyderabad, Hyderabad 500046, Telangana, India.

ACS Omega
|August 29, 2019
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Summary

Researchers developed low-cost filter paper substrates with silver/gold nanoparticles for detecting explosive molecules and dyes using surface-enhanced Raman spectroscopy (SERS). This method offers high sensitivity and reproducibility for trace chemical analysis.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Surface-enhanced Raman spectroscopy (SERS) requires efficient substrates for sensitive detection.
  • Developing cost-effective and versatile SERS substrates is crucial for practical applications.

Purpose of the Study:

  • To fabricate and characterize novel, low-cost filter paper-based SERS substrates.
  • To evaluate the efficacy of these substrates for detecting explosive molecules and dye molecules.

Main Methods:

  • Fabrication of silver (Ag) and gold (Au) nanoparticles (NPs) via femtosecond laser ablation.
  • Preparation of salt-induced aggregated Ag/Au NPs using NaCl.
  • Characterization of NPs and substrates using UV-visible absorption, TEM, and FESEM.
  • Detection of picric acid, 2,4-dinitrotoluene, 3-nitro-1,2,4-triazol-5-one, and methylene blue.

Main Results:

  • Demonstrated efficient SERS detection of multiple explosive molecules (picric acid, DNT, NTO) and methylene blue.
  • Achieved high sensitivity with detection limits in picogram to nanogram range.
  • Showcased superior enhancement, reproducibility, and sensitivity with an optimal NaCl concentration of 50 mM.

Conclusions:

  • Filter paper loaded with salt-induced aggregated Ag/Au NPs serves as a versatile and efficient SERS substrate.
  • The developed substrates offer a promising platform for rapid and sensitive detection of trace analytes.