Continuously Tunable Nucleotide/Lanthanide Coordination Nanoparticles for DNA Adsorption and Sensing
Li Xu1,2, Peipei Zhang1, Yan Liu1
1School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, P. R. China.
This study explores metal-organic coordination polymers (CPs) for DNA adsorption. Researchers found that mixing adenosine monophosphate (AMP) and guanosine monophosphate (GMP) in CPs allows tunable DNA binding, enabling sensitive DNA detection.
Area of Science:
- Materials Science
- Nanotechnology
- Biochemistry
Background:
- Metal-organic coordination polymers (CPs) are versatile materials with applications in biosensing, drug delivery, and catalysis.
- Lanthanide-nucleotide CPs are of interest, but research often focuses on single nucleotide ligands.
- This work investigates mixed nucleotide CPs and their interaction with DNA.
Purpose of the Study:
- To explore the DNA adsorption properties of mixed nucleotide-lanthanide coordination polymers.
- To investigate factors influencing DNA adsorption and desorption.
- To develop a DNA sensor utilizing these CPs.
Main Methods:
- Synthesis of negatively charged coordination polymer nanoparticles using lanthanides and mixed adenosine monophosphate (AMP) and guanosine monophosphate (GMP).
- Adsorption and desorption studies of fluorescently labeled DNA using various agents.
- Characterization of DNA adsorption affinity and development of a DNA sensor based on differential binding of single- and double-stranded DNA.
Main Results:
- Mixed nucleotide CPs formed nanoparticles that adsorbed DNA, requiring salt for negatively charged DNA.
- DNA adsorption capacity and speed correlated with lanthanide properties; lighter lanthanides showed higher capacity.
- Tunable DNA adsorption affinity was achieved by varying the AMP:GMP ratio in CPs, with AMP-rich CPs showing stronger binding.
- Successful desorption of DNA was demonstrated using various agents.
- A DNA sensor was developed with a detection limit of 0.9 nM for target DNA, differentiating between single- and double-stranded DNA.
Conclusions:
- The composition of mixed nucleotide-lanthanide CPs can be controlled to tune DNA adsorption affinity.
- The DNA phosphate backbone plays a crucial role in adsorption, with contributions from hydrogen bonding and bases.
- These tunable CPs offer a novel platform for DNA sensing and potentially other biomolecule interactions.
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