Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

¹H NMR: Complex Splitting

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...

¹H NMR Signal Integration: Overview

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

A protocol for preparing 13C,15N-labeled fungal and plant samples for multidimensional solid-state NMR spectroscopy and dynamic nuclear polarization (DNP) investigations is...

Crystal Field Theory - Octahedral Complexes

Crystal Field Theory - Octahedral Complexes

Crystal Field Theory
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR

The NMR-solution structure of a metallochaperone model peptide with Cu (I) was determined, and a detailed protocol from sample preparation and 1D and 2D data collection to a three-dimensional structure is...

Crystal Field Theory - Tetrahedral and Square Planar Complexes

Crystal Field Theory - Tetrahedral and Square Planar Complexes

Tetrahedral Complexes
Crystal field theory (CFT) is applicable to molecules in geometries other than octahedral. In octahedral complexes, the lobes of the dx2−y2 and dz2 orbitals point directly at the ligands. For tetrahedral complexes, the d orbitals remain in place, but with only four ligands located between the axes. None of the orbitals points directly at the tetrahedral ligands. However, the dx2−y2 and dz2 orbitals (along the Cartesian axes) overlap with the ligands less than the dxy,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The progression of initial symptoms and its relationship with the clinical course in adult-onset Still's disease: from the KEIO-AOSD cohort.

EULAR rheumatology open·2026

Same author

Predictive correlates of arthritis and joint damage in systemic lupus erythematosus: a multinational prospective cohort study.

Rheumatology (Oxford, England)·2026

Same author

RefLaTEA: a robust visualization and analysis framework leveraging background data for enhanced insight.

MethodsX·2026

Same author

Mucocutaneous Disease Activity and Damage Accrual in Systemic Lupus Erythematosus: Analyses From the Asia-Pacific Lupus Collaboration Longitudinal Cohort Study.

Arthritis care & research·2026

Same author

Immune Complexes Shape the Pathogenicity of Autoantibodies in Systemic Autoimmune Diseases: Insights From an FcγRIIIA-Reporter Assay.

Arthritis & rheumatology (Hoboken, N.J.)·2026

Same author

Treatment patterns, treat-to-target goals and clinical outcomes of patients with active lupus nephritis: real-world evidence from a multicentre cohort study.

Arthritis research & therapy·2026

Search research articles

Related Experiment Video

Updated: Jan 20, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

InterSpin: Integrated Supportive Webtools for Low- and High-Field NMR Analyses Toward Molecular Complexity.

Shunji Yamada^1,2, Kengo Ito², Atsushi Kurotani²

¹Graduate School of Bioagricultural Sciences, Nagoya University, 1 Furo-cho, Chikusa-ku, Nagoya, Aichi 464-0810, Japan.

|August 29, 2019

Summary

This summary is machine-generated.

InterSpin offers free web tools and a database for nuclear magnetic resonance (NMR) signal assignment in complex molecules. This system enhances data analysis for food, material, and environmental applications.

More Related Videos

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

1H NMR: Complex Splitting

1H NMR: Complex Splitting

Related Experiment Videos

Last Updated: Jan 20, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

1H NMR: Complex Splitting

1H NMR: Complex Splitting

Area of Science:

Analytical Chemistry
Biophysics
Materials Science

Background:

Nuclear Magnetic Resonance (NMR) spectroscopy is crucial for analyzing molecular structures.
Handling broad spectra from solid-state or low-field NMR presents challenges.
Efficient signal assignment is key for diverse applications in food, materials, and environmental science.

Purpose of the Study:

To introduce InterSpin, a comprehensive resource for NMR data analysis.
To provide accessible web tools and a database for advancing NMR signal assignment.
To support the analysis of small molecules to macromolecules across various NMR techniques.

Main Methods:

Development and evaluation of preprocessing web tools: sensitivity improvement and peak separation.
Creation of the InterSpin Laboratory Information Management System (SpinLIMS) database for standard spectra.
Implementation of molecular assignment tools: SpinMacro for macromolecules and InterAnalysis for small molecules.

Main Results:

Two novel preprocessing tools enhance signal-to-noise ratio and resolve overlapping peaks.
SpinLIMS database provides extensive spectral data searchable under various conditions.
SpinMacro and InterAnalysis facilitate accurate assignment of macromolecules and small molecules, respectively.

Conclusions:

InterSpin provides integrated, freely accessible tools and a database to advance NMR signal assignment.
The system supports diverse applications, from small molecules to macromolecules, in solid and solution states.
Database interoperability through SpinLIMS client software promotes data sharing and scientific discovery.