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Updated: Jan 20, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
Dual-Function Fluorescent Probe for Detection of Hydrogen Sulfide and Water Content in Dimethyl Sulfoxide
Hao Wang1, Yanan Li1, Shaoxiang Yang1
1Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, PR China.
Abstract:
To detect hydrogen sulfide (H2S) and water content in dimethyl sulfoxide, the fluorescent probe (Probe 1) was used, as it not only detects H2S but also detects the water content. After H2S was added into Probe 1, the intensity of fluorescence increased and was up to 1300 times. In case the H2S concentration was in the range 0-20 μM, it was able to be detected by Probe 1, and the limit of detection was 0.851 nM. When Probe 1 and H2S underwent a reaction, the solution color had some changes. These colors changed in terms of the concentration changes of H2S, ranging from colorless to yellow. The Probe 1 test paper only needed to be exposed to hydrogen sulfide gas for 20 s for the color change to occur. Besides, Probe 1-H2S was used to detect water content in dimethyl sulfoxide which ranged from 0 to 100%. The color change of the solution was opposite to that of H2S, ranging from yellow to colorless.
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