[Design of Dyson Flow Cytometry System].

Area of Science:

• Optics and Photonics
• Biomedical Engineering
• Analytical Chemistry

Background:

• Flow cytometry requires systems capable of analyzing multiple fluorescence wavelengths simultaneously.
• Existing systems often face limitations in terms of size and spectral resolution for complex analyses.
• The demand for miniaturized, high-performance spectral analysis in flow cytometry is increasing.

Purpose of the Study:

• To design and develop a compact flow cytometry spectral analysis system.
• To leverage Dyson structure characteristics for enhanced spectral analysis capabilities.
• To meet the growing need for simultaneous multi-wavelength fluorescence detection in small-volume applications.

Main Methods:

• Analysis of Dyson structure characteristics for optical system design.
• Design of a flow cytometry spectral analysis system based on Dyson optics.
• Integration of an Electron Multiplying Charge-Coupled Device (EMCCD) detector for high-speed, high-sensitivity detection.
• Characterization of spectral range (400-800 nm), spectral resolution (<3 nm), and optical performance metrics.

Main Results:

• A flow cytometry spectral analysis system with a spectral range of 400-800 nm was successfully designed.
• The system achieved a spectral resolution of less than 3 nm and a transfer function value above 0.8 at 21 lp/mm.
• The compact system dimensions (83.54 mm×85.60 mm) and performance metrics meet flow cytometry requirements.
• The use of Dyson light splitting and EMCCD detector demonstrated high speed and sensitivity.

Conclusions:

• The developed Dyson-type flow cytometry spectral analysis system offers excellent optical performance and a small footprint.
• The system effectively addresses the need for simultaneous multi-wavelength fluorescence analysis in flow cytometry.
• The innovative application of Dyson optics and EMCCD technology represents a significant advancement in flow cytometry instrumentation.