Role of Fresh Frozen Cartilage in Revision Rhinoplasty

Raja Mohan1, R Raja Shanmuga Krishnan1, Rod J Rohrich1

  • 1From the Dallas Plastic Surgery Institute and Ganga Hospital.

Abstract

Related Concept Videos

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens10:07

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Here, we present a protocol to perform a simple cellular fractionation for the subcellular separation of cytoplasmic and nuclear proteins in human fresh and frozen intestinal...
10.2K
Cresyl Violet Staining of Tissue Sections: A Staining Procedure to Visualize Cartilage and Bones in Frozen Mouse Embryo Sections03:32

Cresyl Violet Staining of Tissue Sections: A Staining Procedure to Visualize Cartilage and Bones in Frozen Mouse Embryo Sections

This video describes cresyl violet staining procedure to visualize bones and cartilage in coronal sections of frozen mouse embryos. This staining technique helps to differentiate bones and cartilage with distinct colors from the surrounding...
2.9K
Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing03:49

Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing

The protocol provides a reliable and optimized approach to the isolation of nuclei from solid tumor specimens for multiome sequencing using the 10x Genomics platform, including recommendations for tissue dissociation conditions, cryopreservation of single-cell suspensions, and assessment of isolated nuclei.
1.7K
Isolation of Nuclei from Fresh Frozen Glioma Tissues: A Method to Obtain Intact Nuclei from Glioma Tumor Samples03:55

Isolation of Nuclei from Fresh Frozen Glioma Tissues: A Method to Obtain Intact Nuclei from Glioma Tumor Samples

This video describes the protocol to isolate single-nuclei from fresh frozen glioma tissue. The isolated single-nuclei can be used as specimens for sequencing techniques such as single-nuclei RNA (snRNA) sequencing and single-nuclei Assay for Transposase-Accessible Chromatin (snATAC) sequencing to obtain deeper insights into inter- and intra-tumor heterogeneity observed in glioma of the central nervous...
2.8K
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones10:11

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard...
10.1K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Intra-tumoral heterogeneity is an inherent feature of tumors, including gliomas. We developed a simple and efficient protocol that utilizes a combination of buffers and gradient centrifugation to isolate single nuclei from fresh frozen glioma tissues for single nucleus RNA and ATAC sequencing...
13.4K