Related Experiment Video

Updated: Jan 20, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

6.9K

A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector

Mireya Martínez-Pérez1, José A Navarro1, Vicente Pallás1

  • 1Department of Molecular and Evolutionary Plant Virology, Instituto de Biología Molecular y Celular de Plantas (IBMCP) (UPV-CSIC), Universitat Politècnica de Valencia, Ingeniero Fausto Elio s/n, 46022 Valencia, Spain.

Virus Research
|August 29, 2019
PubMed

Keywords:
AlfamovirusP12 systemRSS assay

More Related Videos

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

24.9K
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones05:56

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

5.8K

Related Concept Videos

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens10:19

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant...
6.9K
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after...
24.9K
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones05:56

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

An Agrobacterium-based injection (agroinjection) protocol is presented for the inoculation of foxtail mosaic virus and sugarcane mosaic virusclones into maize seedlings. Inoculation in this manner leads to viral infection, virus-induced gene silencing of marker genes, and viral overexpression of...
5.8K
Potato Virus X-Based microRNA Silencing (VbMS) In Potato.11:51

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

We present a detailed protocol for potato virus X (PVX)-based microRNA silencing (VbMS) system to functionally characterize endogenous microRNAs (miRNAs) in potato. Target mimic (TM) molecules of miRNA of interest are integrated into the PVX vector and transiently expressed in potato to silence the target miRNA or miRNA...
3.5K
Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae08:36

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Here we describe a procedure for inhibiting gene function in disease vector mosquitoes through the use of chitosan/interfering RNA nanoparticles that are ingested by...
15.0K
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Nucleic acid degradation in archival tissue, tumor heterogeneity, and a lack of fresh frozen tissue specimens can negatively impact cancer diagnostic services in pathology laboratories worldwide. This manuscript describes the optimization of a panel of biomarkers using a multiplex magnetic bead assay to classify breast...
8.4K