Epilepsy Therapy Goes Viral

This protocol presents robot-assisted stereo electroencephalography (sEEG), a method for the stereotactic implantation of intracerebral electrodes used for invasive seizure monitoring in patients with refractory epilepsy. Techniques incorporating stereotactic robotic guidance for sEEG are safe and precise and may improve surgical efficiency, especially when implanting greater numbers of...

We describe the steps to use our custom designed software for image integration, visualization and planning in epilepsy...

Source: Akil, O., et al., Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane. J. Vis. Exp. (2015)This video demonstrates the procedure of delivering a therapeutic gene into the inner ear of a mouse pup with genetically induced hearing loss. Using an adeno-associated viral (AAV) vector, the gene is injected through the round window membrane, allowing viral particles to enter hair cells, restore glutamate transporter production, and...

Here we provide an overview of the anteromesial temporal lobectomy procedure, used in the treatment of patients with medically refractory temporal lobe epilepsy. In particular, we describe herein, the details of the operative method and related surgical technique, in addition to summarizing the indications and outcomes of the...

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

Subretinal injection is a surgical technique for effective gene delivery to retinal pigment epithelium in the mouse eye. Here we describe an easy and replicable method for subretinal injection of viral vectors to retinal pigment epithelium in experimental...