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Related Experiment Video

Updated: Jan 20, 2026

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
10:05

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Single-Cell Transcriptomic Map of the Human and Mouse Bladders.

Zhenyuan Yu1,2,3,4,5, Jinling Liao2,4,5, Yang Chen1,2,3,4,5

  • 1Institute of Urology and Nephrology.

Journal of the American Society of Nephrology : JASN
|August 30, 2019
PubMed
Summary

Researchers mapped human and mouse bladder cells using single-cell RNA sequencing. This cellular atlas reveals novel bladder cell types and functions, aiding disease research.

Keywords:
bladder cellsepithelial cellsinterstitial cellssingle-cell RNA sequencingsingle-cell transcriptomic map

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Area of Science:

  • Urology
  • Cell Biology
  • Genomics

Background:

  • A comprehensive cellular map of the human bladder is crucial for understanding bladder diseases, including cancer.
  • Investigating cellular origins of benign and malignant bladder conditions requires detailed anatomical knowledge.

Purpose of the Study:

  • To create a single-cell transcriptomic map of healthy human and mouse bladders.
  • To classify bladder cell types and elucidate their functions.
  • To compare cellular homology and heterogeneity between human and mouse bladders.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) was performed on 12,423 human bladder cells and 12,884 mouse bladder cells.
  • Comparative transcriptomic analysis was used to identify conserved and divergent cell types and evolutionary aspects.

Main Results:

  • A single-cell transcriptomic atlas of human and mouse bladders was generated, identifying 16 human and 15 mouse cell clusters.
  • Two novel human bladder cell types were discovered: ADRA2A+/HRH2+ interstitial cells and TNNT1+ epithelial cells.
  • TNNT1+ epithelial cells, potentially involved in bladder emptying, were also found in rat and mouse bladders.

Conclusions:

  • The generated transcriptomic map serves as a valuable resource for bladder research.
  • It facilitates the study of specific cell markers, signaling receptors, and genes.
  • This resource will advance understanding of the relationship between bladder cell types and disease pathogenesis.