Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyclonal IgA in IgA nephropathy.

Y Yasumoto1, T Suga, M Miura

  • 1Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa-ken, Japan.

Annals of the Academy of Medicine, Singapore
|July 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevation drives intraspecific metabolomic differentiation in natural and experimental populations.

Plant biology (Stuttgart, Germany)·2025
Same author

Nightlife clusters of coronavirus disease in Tokyo between March and April 2020.

Epidemiology and infection·2020
Same author

Separation of Neutral Glycoasparagines According to Their Content of cis Diol Groups.

Bioscience, biotechnology, and biochemistry·2016
Same author

Search for lepton flavour violation in the <i>eμ</i> continuum with the ATLAS detector in [Formula: see text]<i>pp</i> collisions at the LHC.

The European physical journal. C, Particles and fields·2015
Same author

Quantification of changes in metamorphopsia and retinal contraction in eyes with spontaneous separation of idiopathic epiretinal membrane.

Eye (London, England)·2013
Same author

Measurement of the W+ W- cross section in sqrt(s) = 7  TeV pp collisions with ATLAS.

Physical review letters·2011

Immunoglobulin A (IgA) nephropathy (IgAN) patients show increased, polyclonal IgA production. This study investigated IgA production in IgAN, revealing polyclonal expansion supports disease heterogeneity.

Area of Science:

  • Immunology
  • Nephrology
  • Molecular Biology

Background:

  • IgA nephropathy (IgAN) is a heterogeneous kidney disease.
  • Understanding the immunological basis of IgAN is crucial for diagnosis and treatment.
  • Aberrant immunoglobulin production may contribute to IgAN pathogenesis.

Purpose of the Study:

  • To investigate the polyclonality of immunoglobulin A (IgA) production in IgA nephropathy (IgAN).
  • To elucidate the immunological aberrations contributing to IgAN.
  • To determine if increased IgA in IgAN is monoclonal or polyclonal.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to quantify IgG, IgA, and IgM levels.
  • Kappa/lambda ratios of immunoglobulins were analyzed in patient sera and lymphocyte culture supernatants.

Related Experiment Videos

  • Lymphocyte cultures were stimulated with pokeweed-mitogen (PWM) to assess B-cell activation.

    • Main Results:

    • Significantly increased IgA levels were observed in the sera and unstimulated lymphocyte supernatants of IgAN patients.
    • No significant changes in IgG or IgM levels were detected between IgAN patients and controls.
    • Kappa/lambda ratios for all immunoglobulins remained unchanged, indicating polyclonal IgA production.

    Conclusions:

    • The increased IgA in IgA nephropathy patients is polyclonal, not monoclonal.
    • Polyclonal IgA expansion supports the concept of IgAN as a heterogeneous disorder.
    • Further research into the specific triggers of polyclonal IgA activation in IgAN is warranted.