Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Obtaining Human Microglia from Adult Human Brain Tissue09:41

Obtaining Human Microglia from Adult Human Brain Tissue

7.8K
This protocol is an efficient, cost effective and robust method of isolating primary microglia from live, adult, human brain tissue. Isolated primary human microglia can serve as a tool for studying cellular processes in homeostasis and disease.
7.8K
Derivation of a Human Brain Organoid with Microglia Development10:34

Derivation of a Human Brain Organoid with Microglia Development

1.9K
We present a protocol to generate a human brain organoid with resident microglia by incorporating Induced pluripotent stem cell (iPSC)-derived hematopoietic progenitor cells (HPCs) into organoid...
1.9K
Magnetic Isolation of Microglia from Mouse Pup Brains04:45

Magnetic Isolation of Microglia from Mouse Pup Brains

853
In this video, we describe a protocol for isolating CD11b cells, including microglia, from mouse pup brains using magnetic-activated cell sorting. The brain tissues are homogenized to obtain single-cell suspensions, which are incubated with anti-CD11b antibody-coated microbeads, followed by a magnetic column-based separation to purify the CD11b+ cell...
853
Isolation of Microglia from the Cortex of an Adult Mouse Brain03:48

Isolation of Microglia from the Cortex of an Adult Mouse Brain

1.2K
The video demonstrates a technique for isolating microglia from the cortex of an adult mouse brain. First, the cortex undergoes segmentation and homogenization to remove neurons and other glial cells, preserving microglia. Following that, the cell suspension is exposed to anti-myelin magnetic microbeads and a magnetic field, effectively separating microglia and myeloid cells from myelin debris and cells containing...
1.2K
Engineering of Human Blood-Induced Microglia-like Cells for Reverse-Translational Brain Research06:12

Engineering of Human Blood-Induced Microglia-like Cells for Reverse-Translational Brain Research

2.0K
This study delineates a novel approach for the establishment of human monocyte-derived microglia-like (iMG) cells that enable the indirect assessment of brain inflammation. This presents a cellular model that may be beneficial to research focusing on potential inflammation of the brain and associated neuropsychiatric...
2.0K
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route05:35

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

3.3K
The protocol presented here allows the transplantation of induced pluripotent stem cell-derived human microglia (iPSMG) into the brain via a transnasal route in immunocompetent mice. The method for the preparation and transnasal transplantation of cells and the administration of cytokine mixture for the maintenance of iPSMG is...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autophagy protein links ageing and Alzheimer disease.

Nature reviews. Neurology·2026
Same author

Veronique Miron: targeting neuroimmunology to protect myelin.

Nature reviews. Neurology·2026
Same author

Qiang Liu: addressing unmet clinical needs through neuroimmune mechanisms.

Nature reviews. Neurology·2026
Same author

Anne-Katrin Pröbstel: B cells, antibodies and microbiota in autoimmune disease.

Nature reviews. Neurology·2026
Same author

Lorenzo's House: shining a light on young-onset dementia.

Nature reviews. Neurology·2026
Same author

Targeting gut pathology is effective in an Alzheimer disease model.

Nature reviews. Neurology·2026

Related Experiment Video

Updated: Jan 20, 2026

Obtaining Human Microglia from Adult Human Brain Tissue
09:41

Obtaining Human Microglia from Adult Human Brain Tissue

Published on: August 30, 2020

7.8K

Mouse brains, human microglia

Ian Fyfe1

  • 1Nature Reviews Neurology, . nrneuro@nature.com.

Nature Reviews. Neurology
|August 30, 2019
PubMed
Summary

No abstract available in PubMed .

More Related Videos

Derivation of a Human Brain Organoid with Microglia Development
10:34

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

1.9K
Magnetic Isolation of Microglia from Mouse Pup Brains
04:45

Magnetic Isolation of Microglia from Mouse Pup Brains

853

Related Experiment Videos

Last Updated: Jan 20, 2026

Obtaining Human Microglia from Adult Human Brain Tissue
09:41

Obtaining Human Microglia from Adult Human Brain Tissue

Published on: August 30, 2020

7.8K
Derivation of a Human Brain Organoid with Microglia Development
10:34

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

1.9K
Magnetic Isolation of Microglia from Mouse Pup Brains
04:45

Magnetic Isolation of Microglia from Mouse Pup Brains

853