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Related Concept Videos

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers09:35

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

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Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10...
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Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells06:26

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The human blood-brain barrier selectively prevents penetration of hydrophilic molecules and pathogens into the brain. Several pathologies, including meningitis and postoperative delirium, are associated with an increased permeability of the blood-brain barrier. Here, we describe an endothelial cell culture model to test the barrier permeability by microbial...
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Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier06:22

Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier

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We present a fluorescence assay to demonstrate that Lucifer Yellow (LY) is a robust marker to determine the apparent paracellular permeability of hCMEC/D3 cell monolayers, an in vitro model of the human blood-brain barrier. We used this assay to determine the kinetics of a confluent monolayer formation in cultured hCMEC/D3 cells.
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Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice06:35

Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice

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Animal models for severe acute pancreatitis enable the study of pathophysiological changes at the initial stage, facilitating observation of the evolution of inflammatory events. Here we provide a protocol for the induction of severe acute biliary pancreatitis by retrograde infusion of sodium taurocholate into the pancreatic duct of anesthetized C57BL/6 mice.
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Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells09:23

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

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The aim of the protocol is to present an optimized procedure for the establishment of an in vitro blood-brain barrier (BBB) model based on primary porcine brain endothelial cells (pBECs). The model shows high reproducibility, high tightness, and is suitable for studies of transport and intracellular trafficking in drug discovery.
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Enema of Traditional Chinese Medicine for Patients with Severe Acute Pancreatitis06:05

Enema of Traditional Chinese Medicine for Patients with Severe Acute Pancreatitis

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An enema is a type of traditional Chinese medicine (TCM) therapy used to treat severe acute pancreatitis (SAP). The therapy involves instilling a TCM liquid into the rectum and colon through the anus to keep the liquid in the intestine, absorbing through the intestinal mucosa. This study aims to standardize the enema procedure for SAP...
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Related Experiment Video

Updated: Jan 20, 2026

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
09:35

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

Published on: February 26, 2018

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Correction to: Mesenchymal stem cells decrease blood-brain barrier permeability in rats with severe acute

Ronggui Lin1, Ming Li2, Meiqin Luo3

  • 11Department of General surgery, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou, Fujian 350001 People's Republic of China.

Cellular & Molecular Biology Letters
|August 30, 2019
PubMed
Summary
This summary is machine-generated.

This study corrects a previous article DOI. The correct DOI is provided for accurate citation and retrieval of scientific information.

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Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
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Last Updated: Jan 20, 2026

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
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Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
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Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
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Area of Science:

  • Bibliometrics
  • Scientific Publishing

Context:

  • Correction of a previously published article DOI.
  • Ensuring accurate citation and retrieval of scientific literature.

Purpose:

  • To provide the correct Digital Object Identifier (DOI) for a specific scientific article.
  • To rectify an error in the original publication's metadata.

Summary:

  • The article DOI: 10.1186/s11658-019-0167-8 has been corrected.
  • This correction ensures proper referencing and access to the research.

Impact:

  • Improved discoverability of the scientific article.
  • Enhanced data integrity within scientific databases and citation indexes.