Related Experiment Video

Updated: Jan 20, 2026

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
12:35

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

Published on: November 10, 2011

19.6K

Hallmarks of Aging in the Liver

Nicholas J Hunt1,2,3, Sun Woo Sophie Kang1,3, Glen P Lockwood1,3

  • 1ANZAC Research Institute, Aging and Alzheimer's Institute, Centre for Education and Research on Ageing, Concord Repatriation General Hospital, Concord, NSW, Australia.

Computational and Structural Biotechnology Journal
|August 30, 2019
PubMed

Keywords:
AMPK, 5′ adenosine monophosphate-activated protein kinaseCR, caloric restrictionEndothelialFOXO, forkhead box OGeneticHSC, hepatic stellate cellHepatocyteIGF-1, insulin like growth factor 1IL-6, interleukin 6IL-8, interleukin 8KC, Küpffer cellLSEC, liver sinusoidal endothelial cellMitochondrial dysfunctionNAD, nicotinamide adenine dinucleotideNAFLD, non-alcoholic fatty liver diseaseNO, nitric oxideNutrient sensing pathwaysPDGF, platelet derived growth factorPGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-αROS, reactive oxygen speciesSIRT1, sirtuin 1SenescenceTNFα, tumor necrosis factor alphaVEGF, vascular endothelial growth factormTOR, mammalian target of rapamycinmiR, microRNAαSMA, alpha smooth muscle actin

More Related Videos

Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice08:58

Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

Published on: July 2, 2020

8.4K
Decellularization and Recellularization of Whole Livers09:24

Decellularization and Recellularization of Whole Livers

Published on: February 4, 2011

22.5K

Related Concept Videos

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion12:35

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and...
19.6K
Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice08:58

Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and...
8.4K
Aging01:26

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
624
Decellularization and Recellularization of Whole Livers09:24

Decellularization and Recellularization of Whole Livers

Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this...
22.5K
Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish09:27

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Here, we present how to generate a non-alcoholic fatty liver disease (NAFLD)-associated Hepatocellular Carcinoma (HCC) zebrafish model to study the impact of cholesterol surplus on liver microenvironment and immune cell...
4.0K
Laparoscopic Anatomical Liver Segment VII Resection with Liver Parenchymal Transection Following a Priority Approach13:57

Laparoscopic Anatomical Liver Segment VII Resection with Liver Parenchymal Transection Following a Priority Approach

This protocol involves a laparoscopic anatomical resection of liver segment VII with liver parenchymal transection following a priority approach. This technique is unaffected by anatomical...
1.1K