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Related Concept Videos

Three-dimensional Optical-resolution Photoacoustic Microscopy08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

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Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging...
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Photoacoustic cystography (PAC) has a great potential to map urinary bladders, a radiation sensitive internal organ in pediatric patients, without using any ionizing radiation or toxic contrast agent. Here we demonstrate the use of PAC for mapping urinary bladders with an injection of optical-opaque tracers in rats in...
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This manuscript describes the novel setup and operating procedure of a photoacoustic microscopy and optical coherence tomography dual-modality system for noninvasive, label-free chorioretinal imaging of larger animals, such as...
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Here a switchable acoustic resolution (AR) and optical resolution (OR) photoacoustic microscopy (AR-OR-PAM) system capable of both high resolution imaging at shallow depth and low resolution deep tissue imaging on the same sample in vivo is...
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A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental...
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Photoacoustic Tomography to Image Blood and Lipids in the Infrarenal Aorta08:38

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Source: Gurneet S. Sangha and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana
Photoacoustic tomography (PAT) is an emerging biomedical imaging modality that utilizes light generated acoustic waves to obtain compositional information from tissue. PAT can be used to image blood and lipid components, which is useful for a wide variety of applications, including cardiovascular and tumor imaging. Currently used imaging techniques have inherent...
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Related Experiment Video

Updated: Jan 20, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

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Published on: May 3, 2011

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Review on practical photoacoustic microscopy.

Seungwan Jeon1, Jongbeom Kim1, Donghyun Lee1

  • 1Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.

Photoacoustics
|August 30, 2019
PubMed
Summary

Photoacoustic microscopy (PAM) offers deep, high-resolution imaging for biological research. This review details methods to optimize PAM systems for specific applications, enhancing resolution, SNR, and speed.

Keywords:
Fast scanningOptoacousticsResolutionSNR

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Area of Science:

  • Biomedical Imaging
  • Optical Physics

Background:

  • Photoacoustic imaging (PAI) provides deep, high-resolution, high-contrast imaging of biological tissues.
  • Photoacoustic microscopy (PAM) is a key PAI technique used in preclinical and clinical research.
  • Various PAM system configurations exist, each with distinct advantages and disadvantages.

Purpose of the Study:

  • To provide practical methods for optimizing PAM system implementation.
  • To enhance resolution, signal-to-noise ratio (SNR), and imaging speed in PAM.
  • To review current preclinical and clinical applications of PAM.

Main Methods:

  • Review of existing PAM system configurations and their performance metrics.
  • Discussion of practical implementation strategies for system optimization.
  • Analysis of application-specific requirements for PAM.

Main Results:

  • Identification of methods to improve PAM resolution, SNR, and imaging speed.
  • Comprehensive overview of PAM's utility in preclinical research.
  • Summary of PAM's current and potential clinical applications.

Conclusions:

  • Optimal PAM system configuration is crucial for maximizing benefits in specific applications.
  • PAM technology continues to evolve, offering significant potential for future biomedical research and diagnostics.