Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

Lu Qi1, Aihong Zhang2, Yu Wang1

  • 11Phase I Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038 China.

BMC Chemistry
|August 30, 2019
PubMed

Related Concept Videos

A Paper-Based Immunoassay for the Detection of Immunoglobulin G04:48

A Paper-Based Immunoassay for the Detection of Immunoglobulin G

The video demonstrates a paper-based immunoassay, involving amine-functionalized cellulose discs cross-linked with glutaraldehyde. Covalent immobilization of IgG-Fc-specific capture antibodies, followed by the introduction of immunoglobulin G and horseradish peroxidase-conjugated detection antibodies, results in a color change confirming the presence of immunoglobulin...
580
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and...
6.7K
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited...
11.9K
Atom Transfer Radical Polymerization of Functionalized Vinyl Monomers Using Perylene as a Visible Light Photocatalyst06:49

Atom Transfer Radical Polymerization of Functionalized Vinyl Monomers Using Perylene as a Visible Light Photocatalyst

A method for the atom transfer radical polymerization of functionalized vinyl monomers using perylene as a visible-light photocatalyst is...
12.4K
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based...
16.3K
Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics03:58

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics

This protocol describes a straightforward process that utilizes convenient plastic micro-molds for simple microembossing operations to fabricate microchannels on nanofibrillated cellulose paper, achieving a minimum width of 200...
2.4K