Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device06:28

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

1.4K
Extracellular vesicles hold immense promise for biomedical applications, but current isolation methods are time-consuming and impractical for clinical use. In this study, we present a microfluidic device that enables the direct isolation of extracellular vesicles from large volumes of biofluids in a continuous manner with minimal steps.
1.4K
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

11.9K
This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited...
11.9K
Isolation of Tissue Extracellular Vesicles from the Liver05:39

Isolation of Tissue Extracellular Vesicles from the Liver

12.4K
This is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue...
12.4K
Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles08:03

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

3.0K
The present protocol describes the isolation of microRNAs from tick salivary glands and purified extracellular vesicles. This is a universal procedure that combines commonly used reagents and supplies. The method also allows the use of a small number of ticks, resulting in quality microRNAs that can be readily sequenced.
3.0K
Isolation and Characterization of Cyanobacterial Extracellular Vesicles08:44

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

6.4K
The present protocol providesdetailed descriptions for isolation, concentration,and characterization of extracellular vesicles from cyanobacterial cultures. Approaches for purifying vesicles from cultures at different scales, trade-offs among methodologies, and considerations for working with field samples are also...
6.4K
Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing12:01

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

19.8K
Extracellular vesicles play important roles in physiological and pathological processes, including coagulation, immune responses, and cancer or as potential therapeutic agents in drug delivery or regenerative medicine. This protocol presents methods for the quantification and size characterization of isolated and non-isolated extracellular vesicles in various fluids using tunable resistive pulse...
19.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative lung tissue functional analysis for pulmonary adverse event risk assessment prior to thoracic radiotherapy.

Physics and imaging in radiation oncology·2026
Same author

Neutrophil to Lymphocyte and Lymphocyte to Monocyte Ratios Predict Improved Survival and Response to Induction Chemotherapy in Locally Advanced Squamous Cell Carcinoma of the Larynx.

Head & neck·2025
Same author

Dynamic release of extracellular particles after opening of the blood-brain barrier predicts glioblastoma susceptibility to paclitaxel.

Nature communications·2025
Same author

Circulating tumor cells as predictive biomarkers in the risk stratification of DCIS: Evidence of early dissemination.

Science advances·2025
Same author

Dosimetry and Toxicity Comparison of Three-Dimensional Conformal Radiation Therapy and Intensity Modulated Radiation Therapy in Locally Advanced Lung Cancer Across a Large Statewide Quality Collaborative.

International journal of radiation oncology, biology, physics·2025
Same author

Reirradiation Special Medical Physics Consultations: Lessons Learned From Nearly 3000 Courses of Treatment.

International journal of radiation oncology, biology, physics·2025

Related Experiment Video

Updated: Jan 20, 2026

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.4K

Multiplex isolation and profiling of extracellular vesicles using a microfluidic DICE device.

Yoon-Tae Kang1, Emma Purcell1, Thomas Hadlock1

  • 1Department of Chemical Engineering and Biointerface Institutes, University of Michigan, 2800 Plymouth Road, NCRC B10-A184, Ann Arbor, MI 48109, USA. snagrath@umich.edu.

The Analyst
|August 30, 2019
PubMed
Summary

This study introduces a new microfluidic device for analyzing extracellular vesicles (EVs) for non-invasive cancer diagnostics. The DICE method efficiently immobilizes and profiles EVs, showing promise for disease monitoring.

More Related Videos

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.9K
Isolation of Tissue Extracellular Vesicles from the Liver
05:39

Isolation of Tissue Extracellular Vesicles from the Liver

Published on: August 21, 2019

12.4K

Related Experiment Videos

Last Updated: Jan 20, 2026

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.4K
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Published on: April 3, 2015

11.9K
Isolation of Tissue Extracellular Vesicles from the Liver
05:39

Isolation of Tissue Extracellular Vesicles from the Liver

Published on: August 21, 2019

12.4K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Biochemistry

Background:

  • Extracellular vesicles (EVs) are crucial biomarkers in liquid biopsies, offering insights into disease states.
  • Challenges exist in unbiased EV enrichment and comprehensive profiling for accurate diagnostics.

Purpose of the Study:

  • To present a novel microfluidic device for immobilizing and analyzing extracellular vesicles (EVs) for multiplexed marker profiling.
  • To develop a simple and efficient method for non-invasive cancer diagnostics using extracellular vesicle characterization.

Main Methods:

  • Developed a microfluidic device with four quadrants for efficient capture of biotinylated EVs.
  • Implemented differentiated immunostaining-based characterization of extracellular vesicles (DICE) for multiplexed protein analysis.
  • Utilized fluorescence staining and controlled sample experiments with cancer cell line EVs.

Main Results:

  • The DICE device achieved 84.4% immobilization efficiency for biotinylated EVs.
  • Successfully facilitated immunofluorescent staining for EVs from non-small cell lung cancer (NSCLC) patients and healthy donors.
  • Demonstrated the device's capability for multiplexed profiling of extracellular proteins.

Conclusions:

  • The DICE device offers a versatile and simple strategy for extracellular vesicle profiling.
  • This method holds potential for advancing non-invasive cancer diagnostics and disease monitoring.
  • The approach can be extended to EVs from various biological origins for broader applications.