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Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes13:36

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

A unique, comprehensive protocol to generate de-sialylated human monocyte-derived dendritic cells (mo-DCs) from isolated peripheral blood mononuclear cells (PBMCs) using a sialidase treatment is presented. Further, methods to assess the phenotypic and functional characterization of mo-DCs and evaluate how sialidase treatment improves the maturation level of mo-DCs are...
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In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells06:28

In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells

Phenol red-free/fetal bovine serum-free medium is a better option than advanced RPMI to eliminate exogenous hormones without altering the normal function of conjunctival goblet cells in the study of sex-based...
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Standardization of Basket Use in Sialendoscopy: A Ten-Year Retrospective Study09:36

Standardization of Basket Use in Sialendoscopy: A Ten-Year Retrospective Study

We present the standardization of the basket use in sialendoscopy for obstructive sialolithiasis in a consecutive ten-year series of patients with obstructive salivary gland, describing localization, stone evaluation, size estimation, choosing basket type, and choosing approach technique (Frontal, Side-to-Side, Back-to-Forward) to achieve a high success rate in its...
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Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic...
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Surgical Correction for Pediatric Epiblepharon and Trichiasis03:59

Surgical Correction for Pediatric Epiblepharon and Trichiasis

Here, we present a protocol for surgical correction for pediatric epiblepharon and...
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Establishment of a Clinic-based Biorepository07:50

Establishment of a Clinic-based Biorepository

Cutaneous tumors are often discarded following Mohs micrographic surgery. A protocol is described here that enables clinical support staff to effectively process and store cutaneous tumor (e.g., squamous cell carcinoma, basal cell carcinoma, and melanoma) samples for downstream laboratory applications without interfering with clinical...
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