Related Experiment Video
Updated: Jan 20, 2026
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Metabolomics by UHPLC-MS: benefits provided by complementary use of Q-TOF and QQQ for pathway profiling
Katrin Freiburghaus1, Carlo Rodolfo Largiadèr1, Christoph Stettler2
1University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Introduction:
Non-targeted metabolic profiling using high-resolution mass spectrometry (HRMS) is a standard approach for pathway identification despite technical limitations.
Objectives:
To assess the performance of combining targeted quadrupole (QQQ) analysis with HRMS for in-depth pathway profiling.
Methods:
Serum of exercising patients with type 1 diabetes (T1D) was profiled using targeted and non-targeted assays.
Results:
Non-targeted analysis yielded a broad unbiased metabolic profile, targeted analysis increased coverage of purine metabolism (twofold) and TCA cycle (three metabolites).
Conclusion:
Our screening strategy combined the benefits of the unbiased full-scan HRMS acquisition with the deeper insight into specific pathways by large-scale QQQ analysis.
More Related Videos
Related Concept Videos
07:34Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
01:44Complementary DNA
09:20Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
11:39Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
03:01Sample Preparation for Metabolomics: A Method to Prepare Cell Samples for Metabolite Profiling
07:12Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS