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Related Concept Videos

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements08:54

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In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
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Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
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Related Experiment Video

Updated: Jan 20, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
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Lipid Profiling Using 1H NMR Spectroscopy.

Miriam Gil1, Sara Samino1, Rubén Barrilero2

  • 1Biosfer Teslab SL, Plaça Prim, Reus, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2019
PubMed
Summary
This summary is machine-generated.

High-throughput lipid profiling using proton nuclear magnetic resonance (1H NMR) enables quantitative analysis of fatty acids, phospholipids, glycerides, and cholesterols in biological samples. This chapter details protocols for sample preparation, NMR experiments, and data quantification with LipSpin software.

Keywords:
1H NMR spectroscopyLipid extractsLipid profiling

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Metabolomics

Background:

  • Lipid profiling is crucial for understanding metabolic regulation in animals.
  • Lipids encompass fatty acids, phospholipids, glycerides, and cholesterols.
  • 1H NMR spectroscopy offers a powerful tool for analyzing complex lipid mixtures.

Purpose of the Study:

  • To provide detailed protocols for NMR-based lipid profiling.
  • To enable high-throughput quantitative analysis of various lipid classes.
  • To demonstrate the application of these methods in biological systems.

Main Methods:

  • Sample preparation techniques for biological matrices (serum, cells, tissues).
  • Standardized 1H NMR experiments for lipid analysis.
  • Quantitative analysis using the specialized LipSpin software tool.

Main Results:

  • Successful quantitative analysis of multiple lipid classes was achieved.
  • 1H NMR experiments proved effective for diverse sample types.
  • The LipSpin software facilitated efficient data processing and quantification.

Conclusions:

  • NMR-based lipid profiling is a robust method for metabolic studies.
  • The presented protocols facilitate high-throughput lipid analysis.
  • This approach is valuable for research in animal metabolism and disease.