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Related Experiment Video

Updated: Jan 20, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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ADAMTS7: Recombinant Protein Expression and Purification.

Rens de Groot1

  • 1Center for Hematology, Imperial College, London, UK. r.degroot@imperial.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2019
PubMed
Summary

ADAMTS7, a protease in cardiovascular and tendon tissues, has unknown functions and substrates. This chapter details its properties and provides a protocol for its study in disease research.

Keywords:
ADAMTS7Chondroitin sulfateIon exchange chromatographyPosttranslational modificationProtein purificationProteoglycanRecombinant protein expressionTransient transfection

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiovascular Science

Background:

  • ADAMTS7 is a secreted protease primarily found in cardiovascular and tendon tissues.
  • Its specific physiological functions and substrates remain largely unknown.
  • Emerging evidence links ADAMTS7 to the development of coronary artery disease.

Purpose of the Study:

  • To introduce the chemical and functional properties of ADAMTS7 domains.
  • To provide a protocol for the recombinant expression and purification of ADAMTS7.
  • To aid scientists studying ADAMTS7's role in cardiovascular disease.

Main Methods:

  • Characterization of ADAMTS7 domains.
  • Development of a recombinant expression and purification protocol.
  • Analysis of posttranslational modifications (glycosylation, chondroitin sulfate attachment).

Main Results:

  • Detailed description of ADAMTS7's chemical and functional domain properties.
  • Established protocol for producing purified recombinant ADAMTS7.
  • Overview of extensive posttranslational modifications affecting ADAMTS7 activity.

Conclusions:

  • ADAMTS7 is a complex protease with significant implications for cardiovascular health.
  • The provided protocol facilitates further research into ADAMTS7's function and substrates.
  • Understanding ADAMTS7 is crucial for elucidating its role in coronary artery disease etiology.