Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Errors in bone blood flow measured with microspheres due to sample preparation technique.

¹Department of Medical Physics, Royal Postgraduate Medical School, London, England.

Clinical Physics and Physiological Measurement : an Official Journal of the Hospital Physicists' Association, Deutsche Gesellschaft Fur Medizinische Physik and the European Federation of Organisations for Medical Physics

|August 1, 1988

Summary

Boiling rabbit carcasses significantly reduces radioactive microsphere content in bones. This method is not recommended for accurate bone blood flow studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A survey of patient informational preferences when choosing between medical and surgical therapy for ulcerative colitis: a sub-study from the DISCUSS project.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2023

Same author

Exploring factors associated with the uneven utilization of telemedicine in Norway: a mixed methods study.

BMC medical informatics and decision making·2017

Same author

Standards for reporting randomized controlled trials in medical informatics: a systematic review of CONSORT adherence in RCTs on clinical decision support.

Journal of the American Medical Informatics Association : JAMIA·2011

Same author

Community photo-triage for skin cancer referrals: an aid to service delivery.

Clinical and experimental dermatology·2010

Same author

Health staff priorities for the future development of telehealth in Western Australia.

Rural and remote health·2009

Same author

Using videotelephony to support paediatric oncology-related palliative care in the home: from abandoned RCT to acceptability study.

Palliative medicine·2008

Area of Science:

Biomedical Engineering
Skeletal Physiology
Experimental Pathology

Background:

Accurate measurement of bone blood flow is crucial for understanding skeletal physiology and disease.
Radioactive microspheres are a common method for assessing tissue perfusion.

Purpose of the Study:

To investigate the impact of carcass boiling on skeletal radioactive microsphere content.
To determine the suitability of boiling as a sample preparation technique for quantitative bone blood flow analysis.

Main Methods:

Radioactive microspheres were injected intracardially into rabbits.
Bones (tibia and femur) were dissected from one limb and boiled in a pressure cooker from the contralateral limb.
Radioactivity in bone samples was quantified to assess microsphere retention.

Related Experiment Videos

Main Results:

Boiling significantly reduced microsphere content: 61% (tibia) and 56% (femur) of control values.
Methodological checks confirmed consistent microsphere distribution between left and right limbs when both were boiled.

Conclusions:

Carcass boiling leads to a substantial loss of microspheres from bone.
Boiling is not a suitable method for sample preparation in quantitative bone blood flow studies.