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Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

The adult structures of Drosophila are derived from sac-like structures called imaginal discs. Analysis of these discs provides insight into many developmental processes including tissue determination, compartment boundary establishment, cell proliferation, cell fate specification, and planar cell polarity. This protocol is used to prepare imaginal discs for light/fluorescent...

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent...

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila

We describe a novel red fluorescent protein (RFP) reporter that is expressed specifically in the Drosophila eye. We detail a methodology for dissection of the eye imaginal disc and how this reporter can be used to aid in the dissection and identification of specific cell types in the developing...

Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia

Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia

Mosaic clone analysis in Drosophila imaginal disc epithelia is a powerful model system to study the genetic and cellular mechanisms of tumorigenesis. Here we describe a protocol to induce tumors in Drosophila wing imaginal discs using the GAL4-UAS system, and introduce a diagnosis method to classify the tumor...

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

Here we describe a protocol to examine the migration of glial cells into the developing Drosophila eye using live microscopic analysis paired with GFP tagged glial cells.

Drosophila Larva Imaginal Disc Dissection: A Method to Observe Developing Epithelia

Drosophila Larva Imaginal Disc Dissection: A Method to Observe Developing Epithelia

When staining Drosophila imaginal discs, often a rough dissection is performed where the discs are exposed but remain attached to the body. Once stained, the discs of interest are isolated and mounted. The example protocol demonstrates a procedure for wing imaginal disc dissection and...

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Related Experiment Video

Updated: Jan 20, 2026

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

Opening up the CPD Imagination

¹Dr. Kitto: Professor, Department of Innovation in Medical Education, Director of Research, Office of Continuing Professional Development, University of Ottawa, Ottawa, Canada, Assistant Professor, Department of Surgery, University of Toronto, Toronto, Canada, and Editor-in-Chief, Journal of Continuing Education in the Health Professions.

The Journal of Continuing Education in the Health Professions

|August 30, 2019

Summary

No abstract available in PubMed .

More Related Videos

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Published on: February 17, 2007

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila

Published on: December 15, 2009

Related Experiment Videos

Last Updated: Jan 20, 2026

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

Published on: February 17, 2007

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila

Published on: December 15, 2009