Related Concept Videos

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct05:18

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct

A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary...
27.1K
3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System08:47

3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System

A method of visualizing and quantifying the 3-dimensional structure of mouse hepatic portal vein or intrahepatic bile duct is described. This resin cast technique can also be applied to other ductal or vascular systems and allows for in situ visualization or quantification of a system's intact communicating...
17.7K
DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis12:39

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

Double resin casting micro-computed tomography, or DUCT, enables visualization, digitalization, and segmentation of two tubular systems simultaneously to facilitate 3D analysis of organ architecture. DUCT combines ex vivo injection of two radiopaque resins followed by micro-computed tomography scanning and segmentation of the tomographic data.
3.7K
Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer08:47

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer

The viscoelastic properties of mucus play a critical role in mucociliary clearance. However, traditional mucus rheological techniques require complex and time-consuming approaches. This study provides a detailed protocol for the use of a benchtop rheometer that can rapidly and reliably perform viscoelastic...
3.9K
Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees05:13

Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees

The present protocol explains a method to feed pesticide-contaminated provisions to the larvae of the solitary bees, Osmia excavata. The procedure examines the ecotoxicity of the pesticide to the larvae of the solitary...
2.6K
Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging05:10

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging

This study presents a 3D imaging method using whole-mount intestinal tissues and multi-photon microscopy to quantify secreted mucus, enabling precise volumetric analysis and visualization of mucus dynamics in response to stimuli like carbamoylcholine...
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