Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Visualizing Intracellular Transport of Cargo in Cultured Astrocytes02:35

Visualizing Intracellular Transport of Cargo in Cultured Astrocytes

332
Source: Creighton, B. A., et al. Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes. J. Vis. Exp. (2019)This video demonstrates the visualization of intracellular cargo transport in cultured astrocytes using a fluorescent acidotropic probe that selectively labels acidic endolysosomal vesicles. Time-lapse imaging and Z-stack acquisition with a confocal microscope allow tracking of fluorescently labeled cargo throughout the three-dimensional cell...
332
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes07:19

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

8.3K
Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and...
8.3K
Epitaxial Nanostructured α-Quartz Films on Silicon: From the Material to New Devices11:34

Epitaxial Nanostructured α-Quartz Films on Silicon: From the Material to New Devices

5.9K
This work presents a detailed protocol for the microfabrication of nanostructured α-quartz cantilever on a Silicon-On-Insulator(SOI) technology substrate starting from the epitaxial growth of quartz film with the dip coating method and then nanostructuration of the thin film via nanoimprint...
5.9K
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier10:16

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

8.0K
A liquid crystal nanoparticle (LCNP) nanocarrier is exploited as a vehicle for the controlled delivery of a hydrophobic cargo to the plasma membrane of living...
8.0K
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis11:09

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

9.9K
Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor...
9.9K
Preparation and Reactivity of Gasless Nanostructured Energetic Materials09:50

Preparation and Reactivity of Gasless Nanostructured Energetic Materials

10.6K
This protocol describes the preparation of gasless nanostructured energetic materials (Ni+Al, Ta+C, Ti+C) using the short-term high-energy ball milling (HEBM) technique. It also describes a high-speed thermal imaging method to study the reactivity of mechanically fabricated nanocomposites. These protocols can be extended to other reactive nanostructured energetic...
10.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Huge analysis of 320,000 careers suggests that productive researchers stay that way.

Nature·2026
Same author

Now is the time for scientific societies to guide global research.

Nature·2026
Same author

Ultrasensitive measurement of brain penetration mechanics and blood vessel rupture with microscale probes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

High-Throughput In Vivo Subcellular Analysis of Gold Nanoparticles for Tumor Mitochondrial Targeting.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Soft photonic skins with dynamic texture and colour control.

Nature·2026
Same author

Nanostraw Electroporation for Temporal RNA Sampling from Living 2D and 3D Cell Culture Systems.

ACS nano·2025

Related Experiment Video

Updated: Jan 20, 2026

Visualizing Intracellular Transport of Cargo in Cultured Astrocytes
02:35

Visualizing Intracellular Transport of Cargo in Cultured Astrocytes

Published on: June 17, 2025

332

Nanostructured Materials for Intracellular Cargo Delivery.

Andy Tay1,2, Nicholas Melosh1

  • 1Department of Materials Science and Engineering , Stanford University , Stanford , California 94305 , United States.

Accounts of Chemical Research
|August 30, 2019
PubMed
Summary

Nanostructures offer a superior alternative for intracellular cargo delivery, overcoming limitations of viral and bulk methods. These nano-scale structures enable precise cellular interaction, improving delivery efficiency and cell health for biomedical applications.

More Related Videos

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
07:19

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Published on: August 28, 2019

8.3K
Epitaxial Nanostructured α-Quartz Films on Silicon: From the Material to New Devices
11:34

Epitaxial Nanostructured α-Quartz Films on Silicon: From the Material to New Devices

Published on: October 6, 2020

5.9K

Related Experiment Videos

Last Updated: Jan 20, 2026

Visualizing Intracellular Transport of Cargo in Cultured Astrocytes
02:35

Visualizing Intracellular Transport of Cargo in Cultured Astrocytes

Published on: June 17, 2025

332
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
07:19

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Published on: August 28, 2019

8.3K
Epitaxial Nanostructured α-Quartz Films on Silicon: From the Material to New Devices
11:34

Epitaxial Nanostructured α-Quartz Films on Silicon: From the Material to New Devices

Published on: October 6, 2020

5.9K

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Cell Biology

Background:

  • Intracellular cargo delivery is crucial for gene editing and biologics therapy, facing challenges with traditional viral and bulk methods.
  • Primary cells like stem and immune cells are vital for therapies but poorly compatible with existing delivery techniques.
  • Nanostructures offer a promising alternative due to their size and localized interaction capabilities.

Purpose of the Study:

  • To review the advancements in nanostructure-mediated intracellular cargo delivery.
  • To compare nanostructures with conventional delivery methods like viruses and electroporation.
  • To propose standardized metrics for reporting delivery efficiency and cell perturbation.

Main Methods:

  • Review of historical developments in nanostructure-cell interactions.
  • Analysis of nano-bio interaction mechanisms and field-coupling strategies (electric/magnetic).
  • Comparative assessment of nanostructures versus viral, biochemical, and bulk electroporation techniques.

Main Results:

  • Nanostructures provide a powerful alternative to conventional methods, minimally perturbing cells.
  • They enable precise, localized interaction, facilitating stronger stimuli application.
  • Successful interfacing with diverse cells (immune cells, cardiomyocytes) for in vitro and in vivo applications.

Conclusions:

  • Nanostructures represent a significant advancement in intracellular cargo delivery, offering improved efficiency and cell viability.
  • Further research and standardization of reporting metrics are needed to fully harness their therapeutic potential.
  • Nanostructure-mediated delivery holds promise for future cell-based therapies and biomedical applications.